Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan.
Analyst. 2020 Oct 26;145(21):6895-6900. doi: 10.1039/d0an00912a.
In this study, we developed bipolar electrochemical microscopy (BEM) using a closed bipolar electrode (cBPE) array with an electrochemiluminescence (ECL) detecting system. Because cBPEs are not directly connected to a detector, high spatio-temporal resolution imaging can be achieved by fabricating a microelectrode array in which each electrode point is arranged in a short interval. A cBPE array with individual cBPEs arranged in 41 μm intervals was successfully fabricated by depositing gold in the pores of a track-etched membrane using electroless plating. Using BEM with the cBPE array, which has a higher density of electrode points than the conventional multi-electrode array, we effectively demonstrated the imaging of [Fe(CN)6]3- diffusion and the respiratory activity of MCF-7 spheroids with high spatio-temporal resolution.
在这项研究中，我们使用带有电化学发光（ECL）检测系统的封闭双极电极（cBPE）阵列开发了双极电化学显微镜（BEM）。由于 cBPE 与检测器没有直接连接，因此通过制造微电极阵列可以实现高时空分辨率成像，其中每个电极点都排列在短间隔内。通过使用无电镀在径迹蚀刻膜的孔中沉积金，成功制造了具有 41μm 间隔的单个 cBPE 排列的 cBPE 阵列。使用具有比传统多电极阵列更高的电极点密度的 cBPE 阵列的 BEM，我们有效地演示了[Fe（CN）6]3-扩散和 MCF-7 球体呼吸活性的高时空分辨率成像。
