Adamu Betelhem Anteneh, Emiru Yohannes Kelifa, Sintayehu Biruk, Araya Ephrem Mebrhatu, Periasamy Gomathi, Gebrelibanos Hiben Mebrahtom
Department of Pharmacognosy, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia.
J Exp Pharmacol. 2020 Aug 5;12:221-231. doi: 10.2147/JEP.S258566. eCollection 2020.
Liver diseases contribute a prominent global burden of mortality and morbidity. The current therapies of liver diseases have numerous limitations including severe adverse effects. This denotes that new more effective, safer, and cheaper drugs are required and medicinal plants used in traditional medicines often offer ideal opportunities. Accordingly, the present study aimed to evaluate the in vivo hepatoprotective and in vitro radical scavenging activities of dried rhizome extracts of (), which is traditionally claimed to provide hepatoprotection.
Hepatoprotective activity of extracts was evaluated using carbon tetrachloride (CCl)-induced liver injury in mice. Pre- and post-treatment models were employed to test the effect of the extracts and silymarin (standard drug). Serum biochemical markers and liver histopathology were used as parameters to evaluate hepatoprotective activities whereas in vitro radical scavenging activity was tested by 2, 2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay.
Oral administration of CCl (1 ml/kg) significantly (<0.001) raised the serum levels of liver enzyme markers compared to the normal control group. Pre-treatment with 125, 250, and 500 mg/kg of extract reduced the serum level of CCl-induced rise in liver enzyme markers with the highest reduction observed at a dose of 500 mg/kg. Likewise, in the post-treatment model, the crude extract and butanol fraction at dose 500 mg/kg reduced levels of liver enzymes. Histopathological examinations revealed lesser liver damage of extract-treated mice compared to the toxic (CCl-treated) controls. The in vitro radical scavenging activity of the different extracts showed concentration-dependent radical scavenging activity. Thus, the results of this study may justify the traditional use of the plant as a hepatoprotective agent.
Results of serum biochemical markers and histopathological examinations of CCl-induced mice models, in the present study, show the hepatoprotective potential of extracts from the rhizome of .
肝脏疾病在全球造成了显著的死亡和发病负担。目前的肝脏疾病治疗方法有许多局限性，包括严重的不良反应。这表明需要新的、更有效、更安全且更便宜的药物，而传统医学中使用的药用植物往往提供了理想的机会。因此，本研究旨在评估传统上声称具有肝脏保护作用的（）干燥根茎提取物的体内肝脏保护作用和体外自由基清除活性。
使用四氯化碳（CCl）诱导的小鼠肝损伤来评估提取物的肝脏保护活性。采用预处理和后处理模型来测试提取物和水飞蓟宾（标准药物）的效果。血清生化标志物和肝脏组织病理学被用作评估肝脏保护活性的参数，而体外自由基清除活性则通过2,2-二苯基-1-苦基肼（DPPH）测定法进行测试。
与正常对照组相比，口服CCl（1 ml/kg）显著（<0.001）提高了肝酶标志物的血清水平。用125、250和500 mg/kg的提取物进行预处理可降低CCl诱导的肝酶标志物血清水平升高幅度，在500 mg/kg剂量下观察到的降低幅度最大。同样，在后处理模型中，500 mg/kg剂量的粗提取物和丁醇馏分降低了肝酶水平。组织病理学检查显示，与有毒（CCl处理）对照组相比，提取物处理的小鼠肝脏损伤较小。不同提取物的体外自由基清除活性呈现浓度依赖性自由基清除活性。因此，本研究结果可能证明该植物作为肝脏保护剂的传统用途是合理的。
本研究中CCl诱导的小鼠模型的血清生化标志物和组织病理学检查结果显示了该根茎提取物的肝脏保护潜力。
