Zhao Peng, Chen Sha, Zhou Jun, Zhang Suyi, Huo Danqun, Hou Changjun
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, PR China.
Liquor Making Biology Technology and Application of Key Laboratory of Sichuan Province, College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000, PR China.
Anal Chim Acta. 2020 Sep 1;1128:90-98. doi: 10.1016/j.aca.2020.06.008. Epub 2020 Jun 17.
Herein, a kind of novel hemin-based metal organic frameworks (Fe-hemin-MOFs) with unique peroxidase-like bioactivity was developed for the first time. The synthesized Fe-hemin-MOFs exhibited satisfactory catalytic activity toward hydrogen peroxide (HO). When it was further supported on Chitosan-reduced graphene oxide (CS-rGO), amplified electrochemical signal could be obtained. The Fe-hemin-MOFs/CS-rGO composite was used to construct a novel HO electrochemical sensor. The electrocatalytic reduction of HO displayed two segments linearity range from 1 to 61 μM and 61-1311 μM, as well as a low detection limit of 0.57 μM. Furthermore, the proposed sensor was successfully used for real-time monitoring of HO released from living cells, which extended the practical application of MOFs-based sensors in monitoring the pathological process in living cells.
在此，首次开发了一种具有独特类过氧化物酶生物活性的新型基于血红素的金属有机框架材料（Fe-血红素-MOFs）。合成的Fe-血红素-MOFs对过氧化氢（H₂O₂）表现出令人满意的催化活性。当它进一步负载在壳聚糖还原氧化石墨烯（CS-rGO）上时，可以获得放大的电化学信号。Fe-血红素-MOFs/CS-rGO复合材料用于构建新型H₂O₂电化学传感器。H₂O₂的电催化还原显示出两个线性范围，分别为1至61μM和61 - 1311μM，以及0.57μM的低检测限。此外，所提出的传感器成功用于实时监测活细胞释放的H₂O₂，这扩展了基于MOFs的传感器在监测活细胞病理过程中的实际应用。
