Wildlife Health Services, Zoological Society of London, London, UK
Wildlife Health Services, Zoological Society of London, London, UK.
Vet Rec. 2020 Nov 14;187(10):e86. doi: 10.1136/vr.105682. Epub 2020 Aug 21.
When suspect were cultured from fish at ZSL London Zoo, investigations were carried out to determine whether they were possible causes of cholera.
Bacterial culture was carried out on fish examined postmortem and colonies were identified using standard techniques including the API 20NE biochemical test kits. Suspect isolates were submitted to the Public Health England laboratory for additional testing. Separately, a number of fish were submitted for routine histopathology.
On 13 occasions between 2014 and 2018, suspected were cultured from individuals of eight different freshwater fish species. Archived cultures for eight of these (from six different fish species) were investigated and seven isolates (from five fish species) were confirmed as , but all were non-O1, non-O139 strains. Whole-genome sequencing showed that the five fish species had unique multilocus sequence types (three isolates from were identical), all of which were genetically distant from human isolates.
There was no evidence that these isolates could cause cholera. Histopathological changes consistent with vibriosis were seen in several fish, suggesting that were causing the disease, but there were also concurrent infections or predisposing stress factors.
当 ZSL 伦敦动物园从鱼类中检测到弧菌时，进行了调查，以确定它们是否可能是霍乱的病因。
对死后检查的鱼类进行细菌培养，并使用标准技术（包括 API 20NE 生化测试试剂盒）对菌落进行鉴定。疑似分离株被提交给英国公共卫生实验室进行进一步检测。此外，还提交了一些鱼类进行常规组织病理学检查。
2014 年至 2018 年期间，共有 13 次从 8 种不同淡水鱼类个体中培养出疑似弧菌。对其中 8 个（来自 6 种不同鱼类）的存档培养物进行了调查，确认了 7 个分离株（来自 5 种鱼类）为弧菌，但均非 O1 群和 O139 群。全基因组测序显示，这 5 种鱼类具有独特的多位点序列类型（来自 3 株的序列完全相同），均与人类分离株在遗传上存在较大差异。
没有证据表明这些分离株会引起霍乱。在一些鱼类中观察到与弧菌病一致的组织病理学变化，表明弧菌正在引起疾病，但也存在并发感染或易感的应激因素。
