Bican P, Spahni A
Swiss Federal Dairy Research Institute, Liebefeld-Bern.
Experientia. 1988 Apr 15;44(4):348-51. doi: 10.1007/BF01961279.
More than 99% of L. lactis cells have been converted to protoplasts upon digestion of cell walls with mutanolysin (N-(acetyl)muramidase). Functional protoplasts were obtained even with the lowest level of the enzyme that was used (0.1 U.ml-1 of the cell suspension) and after incubation at 37 degrees C for 2 min. The regeneration of the polymerized cell wall appears to be induced by a cell homogenate of the same organism.
在用变溶菌素（N-（乙酰）胞壁酸酶）消化细胞壁后，超过99%的乳酸乳球菌细胞已转化为原生质体。即使使用最低水平的酶（细胞悬液为0.1 U.ml-1）并在37℃孵育2分钟后，仍可获得功能性原生质体。聚合细胞壁的再生似乎是由同一生物体的细胞匀浆诱导的。
