Do Thien Bui-Thuan, Nguyen Tien Ngoc-Thuy, Ho Minh Hieu, Nguyen Nghi Thi-Phuong, Do Thai Minh, Vo Dai Tan, Hua Ha Thi-Ngoc, Phan Thang Bach, Tran Phong A, Nguyen Hoai Thi-Thu, Vo Toi Van, Nguyen Thi-Hiep
Department of Tissue Engineering and Regenerative Medicine, School of Biomedical Engineering, International University, Ho Chi Minh City 700000, Vietnam.
Vietnam National University, Ho Chi Minh City 700000, Vietnam.
Polymers (Basel). 2021 Sep 15;13(18):3116. doi: 10.3390/polym13183116.
(1) Background: Wounds with damages to the subcutaneous are difficult to regenerate because of the tissue damages and complications such as bacterial infection. (2) Methods: In this study, we created burn wounds on pigs and investigated the efficacy of three biomaterials: polycaprolactone-gelatin-silver membrane (PCLGelAg) and two commercial burn dressings, Aquacel Ag and UrgoTul silver sulfadiazine. In vitro long-term antibacterial property and in vivo wound healing performance were investigated. Agar diffusion assays were employed to evaluate bacterial inhibition at different time intervals. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time-kill assays were used to compare antibacterial strength among samples. Second-degree burn wounds in the pig model were designed to evaluate the efficiency of all dressings in supporting the wound healing process. (3) Results: The results showed that PCLGelAg membrane was the most effective in killing both Gram-positive and Gram-negative bacteria bacteria with the lowest MBC value. All three dressings (PCLGelAg, Aquacel, and UrgoTul) exhibited bactericidal effect during the first 24 h, supported wound healing as well as prevented infection and inflammation. (4) Conclusions: The results suggest that the PCLGelAg membrane is a practical solution for the treatment of severe burn injury and other infection-related skin complications.
(1) 背景：由于组织损伤以及诸如细菌感染等并发症，皮下组织受损的伤口难以再生。(2) 方法：在本研究中，我们在猪身上制造烧伤伤口，并研究了三种生物材料的疗效：聚己内酯 - 明胶 - 银膜（PCLGelAg）以及两种商用烧伤敷料，即爱康肤银敷料（Aquacel Ag）和优格涂磺胺嘧啶银敷料（UrgoTul silver sulfadiazine）。研究了它们的体外长期抗菌性能和体内伤口愈合性能。采用琼脂扩散试验评估不同时间间隔的细菌抑制情况。使用最低抑菌浓度（MIC）、最低杀菌浓度（MBC）和时间 - 杀菌试验来比较各样本之间的抗菌强度。在猪模型中设计二度烧伤伤口，以评估所有敷料在支持伤口愈合过程中的效果。(3) 结果：结果表明，PCLGelAg膜对革兰氏阳性菌和革兰氏阴性菌的杀菌效果最为显著，其MBC值最低。所有三种敷料（PCLGelAg、爱康肤银敷料和优格涂磺胺嘧啶银敷料）在最初24小时内均表现出杀菌作用，支持伤口愈合并预防感染和炎症。(4) 结论：结果表明，PCLGelAg膜是治疗严重烧伤损伤和其他感染相关皮肤并发症的一种实用解决方案。
