平溃灌肠剂通过调节大鼠炎症因子、肠道双歧杆菌和肠黏膜屏障减轻三硝基苯磺酸诱导的溃疡性结肠炎
Pingkui Enema Alleviates TNBS-Induced Ulcerative Colitis by Regulation of Inflammatory Factors, Gut Bifidobacterium, and Intestinal Mucosal Barrier in Rats.
作者信息
Yun Hai-Feng, Liu Rui, Han Dan, Zhao Xin, Guo Jin-Wei, Yan Feng-Jiao, Zhang Chuan, Sun Hong-Wen, Liang Guo-Qiang, Zhang Guo-Xing
机构信息
Department of Internal Medicine, Suzhou Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, 18 Yang-Su Road, Suzhou 215003, China.
Suzhou Academy of Wumen Chinese Medicine, Suzhou Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, 18 Yang-Su Road, Suzhou 215003, China.
出版信息
Evid Based Complement Alternat Med. 2020 Aug 5;2020:3896948. doi: 10.1155/2020/3896948. eCollection 2020.
BACKGROUND
Ulcerative colitis (UC) is a chronic recurrent inflammation of the colon, and clinical outcome of UC is still unsatisfied. Pingkui enema, a traditional Chinese medicine prescription, has been safely applied for the treatment of diarrhea and dysentery in clinic for many years. However, its mechanism is still elusive. The present study is designed to investigate the effect of Pingkui enema on trinitrobenzene sulfonic acid- (TNBS-) induced ulcerative colitis (UC) and possible mechanism in rats.
METHODS
UC was induced by intracolonic instillation of TNBS in male Sprague-Dawley rats, which were treated with different dosages of Pingkui enema (low, medium, and high) or sulfasalazine for ten days. Survival rate was calculated. A clinical disease activity score was evaluated. Histological colitis severity was analyzed by hematoxylin-eosin (HE) staining. Content of Bifidobacterium in intestinal tissue was analyzed by RT-PCR. Concentration of IL-8, IL-13, TNF-, -lactic acid (-LA), and diamine oxidase (DAO) in serum and contents of adhesin and receptor of Bifidobacterium adhesion in rat intestinal mucus were measured by ELISA.
RESULTS
The results showed that Pingkui enema treatment with high dosage markedly improved the survival rate compared with untreated and sulfasalazine treated groups. All dosages of Pingkui enema reduced pathological score. High dosage of Pingkui enema and sulfasalazine treatments significantly reduced the serum concentration of IL-8, TNF-, -LA, and DAO and markedly increased the serum concentration of IL-13. In addition, high-dose Pingkui enema and sulfasalazine treatments increased gut content of Bifidobacterium, gut mucus expressions of adhesin, and adhesin receptor of Bifidobacterium.
CONCLUSIONS
Pingkui enema has therapeutic effect on TNBS-induced UC, and possible mechanism may be via regulation of gut probiotics (Bifidobacterium) and inflammatory factors and protection of intestinal mucosal barrier.
背景
溃疡性结肠炎（UC）是一种结肠的慢性复发性炎症，UC的临床治疗效果仍不尽人意。平溃灌肠剂是一种中药方剂，多年来已在临床上安全应用于治疗腹泻和痢疾。然而，其作用机制仍不清楚。本研究旨在探讨平溃灌肠剂对三硝基苯磺酸（TNBS）诱导的大鼠溃疡性结肠炎（UC）的影响及其可能的机制。
方法
通过向雄性Sprague-Dawley大鼠结肠内注入TNBS诱导UC，将其分为不同剂量（低、中、高）的平溃灌肠剂组或柳氮磺胺吡啶组，治疗10天。计算生存率。评估临床疾病活动评分。通过苏木精-伊红（HE）染色分析组织学结肠炎严重程度。通过RT-PCR分析肠道组织中双歧杆菌的含量。采用ELISA法检测血清中白细胞介素-8（IL-8）、白细胞介素-13（IL-13）、肿瘤坏死因子-α（TNF-α）、乳酸（-LA）和二胺氧化酶（DAO）的浓度，以及大鼠肠道黏液中双歧杆菌黏附素和受体的含量。
结果
结果显示，与未治疗组和柳氮磺胺吡啶治疗组相比，高剂量平溃灌肠剂治疗显著提高了生存率。所有剂量的平溃灌肠剂均降低了病理评分。高剂量平溃灌肠剂和柳氮磺胺吡啶治疗显著降低了血清中IL-8、TNF-α、-LA和DAO的浓度，并显著提高了血清中IL-13的浓度。此外，高剂量平溃灌肠剂和柳氮磺胺吡啶治疗增加了肠道中双歧杆菌的含量、肠道黏液中双歧杆菌黏附素的表达以及黏附素受体的表达。
结论
平溃灌肠剂对TNBS诱导的UC具有治疗作用，其可能的机制可能是通过调节肠道益生菌（双歧杆菌）和炎症因子以及保护肠道黏膜屏障。
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