Waszczuk Mairique, Bianchi Sara Elis, Martiny Simony, Pittol Vanessa, Lacerda Denise S, Araújo Alex Sander da Rosa, Bassani Valquiria Linck
Laboratório de Desenvolvimento Galênico, Programa de Pós Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 6° andar, CEP: 90610-000, Porto Alegre, RS, Brazil.
Anal Methods. 2020 Sep 17;12(35):4310-4318. doi: 10.1039/d0ay00989j.
Pterostilbene is a natural constituent with numerous preventive and therapeutic properties used for treating a wide range of human diseases. It has been isolated from blueberries in high concentrations. A versatile specific-stability indicating liquid chromatography method for reliable quantitative determination of pterostilbene in blueberry extracts was developed. In the stability-indicating analysis, pterostilbene samples were exposed to stress conditions: temperature, UV light, and oxidative, acid and alkaline media. The robustness of the method was evaluated using a Box-Behnken experimental design. Pterostilbene was stable in acid and alkaline media, but unstable when exposed to oxidation, high temperature, and UV light. Using the proposed method, it was demonstrated that degradation products did not interfere in the analyte analysis. The method revealed to be simple, fast, selective, linear, precise and accurate for the quantitative analysis of pterostilbene in Vaccinium myrtillus extract. Besides being a specific-stability indicating method, it demonstrated to be applicable to quantify pterostilbene in pterostilbene:β-cyclodextrin complexes and pterostilbene:β-cyclodextrin:hydrophilic polymer ternary systems. These findings are relevant for the development of food, pharmaceutical or cosmetic products containing pterostilbene or blueberry extracts.
紫檀芪是一种具有多种预防和治疗特性的天然成分，可用于治疗多种人类疾病。它在蓝莓中以高浓度分离出来。开发了一种通用的特定稳定性指示液相色谱法，用于可靠地定量测定蓝莓提取物中的紫檀芪。在稳定性指示分析中，将紫檀芪样品置于压力条件下：温度、紫外线、氧化、酸性和碱性介质。使用Box-Behnken实验设计评估该方法的稳健性。紫檀芪在酸性和碱性介质中稳定，但在暴露于氧化、高温和紫外线时不稳定。使用所提出的方法，证明降解产物不会干扰分析物分析。该方法对于越橘提取物中紫檀芪的定量分析显示出简单、快速、选择性、线性、精确和准确。除了是一种特定稳定性指示方法外，它还证明适用于定量紫檀芪：β-环糊精复合物和紫檀芪：β-环糊精：亲水性聚合物三元体系中的紫檀芪。这些发现对于开发含有紫檀芪或蓝莓提取物的食品、药品或化妆品具有重要意义。
