Madden S L, Creasy C L, Srinivas V, Fawcett W, Bergman L W
Department of Biological Sciences, University of Maryland, Baltimore County, Catonsville 21228.
Nucleic Acids Res. 1988 Mar 25;16(6):2625-37. doi: 10.1093/nar/16.6.2625.
In yeast, the repression of acid phosphatase under high phosphate growth conditions requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis and level of a PHO80-LacZ fusion protein is independent of the level of phosphate in the growth medium. Disruption of the PHO80 gene is a non-lethal event and causes a derepressed phenotype, with acid phosphatase levels which are 3-4 fold higher than the level found in derepressed wild type cells. Furthermore, over-expression of the PHO80 gene causes a reduction in the level of acid phosphatase produced under derepressed growth conditions. Finally, we have cloned, localized and sequenced a temperature-sensitive allele of PHO80 and found the phenotype to be due to T to C transition causing a substitution of a Ser for a Leu at amino acid 163 in the protein product.
在酵母中，高磷酸盐生长条件下酸性磷酸酶的抑制需要反式作用因子PHO80。我们已经确定了PHO80基因的DNA序列，发现它编码一个由293个氨基酸组成的蛋白质。通过Northern分析和PHO80-LacZ融合蛋白水平测定，PHO80基因的表达与生长培养基中磷酸盐的水平无关。PHO80基因的破坏是一个非致死事件，并导致去阻遏表型，酸性磷酸酶水平比去阻遏野生型细胞中的水平高3至4倍。此外，PHO80基因的过表达导致在去阻遏生长条件下产生的酸性磷酸酶水平降低。最后，我们克隆、定位并测序了PHO80的一个温度敏感等位基因，发现该表型是由于T到C的转变导致蛋白质产物中第163位氨基酸的丝氨酸被亮氨酸取代。
