Räsänen L, Arvilommi H
Immunology. 1977 Jun;32(6):843-7.
We studied whether reactions at the lymphocyte membrane receptors for complement, immunoglobulin or mitogens would induce lymphokine production. Human peripheral blood mono-nuclear cells were stimulated by C3- or Fc-receptor reactions and with phytohaemagglutinin (PHA) or lipopolysaccharide (LPS) and cultured for 3 or 4 days. C3- and Fc-receptor reactions were brought about by rosette formation with red cells coated with human complement or antibody. Culture supernatants were assayed for migration inhibitory activity with human leucocytes by the leucocyte-migration-in-agarose test. On the average, no migration inhibitory activity could be detected in cultures stimulated by C3- or Fc-rosette formation, mean migration indices (MI) being 99-2 and 96-1, respectively. Of the mitogens, PHA induced distinct lymphokine synthesis (mean MI 60-2) while the mean MI with LPS varied from 97-9-69-8, depending on the mitogen preparation used and the conditions of culture. We conclude that PHA and LPS are able to activate human lymphocytes into elaboration of migration inhibitory factors whilst reactions at C3- or Fc-receptors fail to do so.
我们研究了补体、免疫球蛋白或丝裂原的淋巴细胞膜受体处的反应是否会诱导淋巴因子的产生。用人外周血单核细胞通过C3或Fc受体反应以及用植物血凝素（PHA）或脂多糖（LPS）进行刺激，并培养3或4天。C3和Fc受体反应是通过与包被有人补体或抗体的红细胞形成玫瑰花结来实现的。通过琼脂糖白细胞迁移试验用人白细胞检测培养上清液的迁移抑制活性。平均而言，在由C3或Fc玫瑰花结形成刺激的培养物中未检测到迁移抑制活性，平均迁移指数（MI）分别为99 - 2和96 - 1。在丝裂原中，PHA诱导明显的淋巴因子合成（平均MI为60 - 2），而LPS的平均MI根据所用丝裂原制剂和培养条件在97 - 9至69 - 8之间变化。我们得出结论，PHA和LPS能够激活人淋巴细胞以产生迁移抑制因子，而C3或Fc受体处的反应则不能。
