Instituto de Fisiología Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2000, Rosario, Argentina.
Instituto de Biología Molecular y Cellular de Rosario, CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina.
Biochimie. 2020 Oct;177:127-131. doi: 10.1016/j.biochi.2020.08.014. Epub 2020 Aug 22.
A-kinase anchoring protein 350 (AKAP350) is a centrosomal/Golgi scaffold protein, critical for the regulation of microtubule dynamics. AKAP350 recruits end-binding protein 1 (EB1) to the centrosome in mitotic cells, ensuring proper spindle orientation in epithelial cells. AKAP350 also interacts with p150, the main component of the dynactin complex. In the present work, we found that AKAP350 localized p150 to the spindle poles, facilitating p150/EB1 interaction at these structures. Our results further showed that the decrease in AKAP350 expression reduced p150 localization at astral microtubules and impaired the elongation of astral microtubules during anaphase. Overall, this study provides mechanistic data on how microtubule regulatory proteins gather to define microtubule dynamics in mitotic cells.
A-激酶锚定蛋白 350（AKAP350）是一种中心体/高尔基体支架蛋白，对于微管动力学的调节至关重要。AKAP350 在有丝分裂细胞中招募末端结合蛋白 1（EB1）到中心体，确保上皮细胞中纺锤体的正确取向。AKAP350 还与动力蛋白复合物的主要成分 p150 相互作用。在本工作中，我们发现 AKAP350 将 p150 定位到纺锤体两极，促进这些结构处的 p150/EB1 相互作用。我们的结果还表明，AKAP350 表达的减少降低了星体微管上的 p150 定位，并损害了有丝分裂后期星体微管的伸长。总的来说，这项研究提供了关于微管调节蛋白如何聚集以定义有丝分裂细胞中微管动力学的机制数据。
