Univ Montpellier, INSERM U1203, EmbryoPluripotency, Montpellier, France.
IRMB, Univ Montpellier, INSERM, Montpellier, France.
Hum Fertil (Camb). 2022 Apr;25(2):356-368. doi: 10.1080/14647273.2020.1807065. Epub 2020 Aug 26.
This is a retrospective study to evaluate if the miRNome profile of endometrium samples collected during the implantation window predicts Assisted Reproduction Technology (ART) outcomes. We first investigated the endometrial miRNome profile according to the receptivity status in 20 patients with repeated implantation failures (RIF) (discovery cohort). After customized embryo transfer, the miRNome profiles of receptive patients with a positive or negative β-hCG, and with early miscarriage or live birth were analysed. Some differentially expressed miRNAs were selected for validation by RT-qPCR in endometrial samples from 103 RIF patients (validation cohort). Analysis of the different miRNome profiles identified endometrial receptivity, implantation failure, and early miscarriage-associated miRNA signatures that included 11, 261, and 76 miRNAs, respectively. However, only four miRNAs associated with the endometrial receptivity status (miR-455-3p and miR-4423-3p) and implantation failure (miR-152-3p and miR-155-5p) were significantly validated in endometrial samples. The miRNome profile of endometrial tissues during the implantation window can predict the pregnancy outcome. These data are crucial for opening new perspectives to predict implantation failure and consequently, to increase ART success.
这是一项回顾性研究，旨在评估在着床窗口期采集的子宫内膜样本的 miRNA 组谱是否可以预测辅助生殖技术 (ART) 的结果。我们首先在 20 名反复着床失败 (RIF) 患者中（发现队列）根据接受状态调查了子宫内膜的 miRNA 组谱。在定制胚胎移植后，分析了接受治疗且 β-hCG 阳性或阴性、有早期流产或活产的患者的 miRNA 组谱。选择一些差异表达的 miRNA 通过 RT-qPCR 在 103 名 RIF 患者的子宫内膜样本中进行验证（验证队列）。不同的 miRNA 组谱分析确定了与子宫内膜接受状态、着床失败和早期流产相关的 miRNA 特征，分别包括 11、261 和 76 个 miRNA。然而，只有与子宫内膜接受状态（miR-455-3p 和 miR-4423-3p）和着床失败（miR-152-3p 和 miR-155-5p）相关的 4 个 miRNA 在子宫内膜样本中得到了显著验证。着床窗口期子宫内膜组织的 miRNA 组谱可以预测妊娠结局。这些数据对于开辟新的视角来预测着床失败，从而提高 ART 成功率至关重要。
