Shakhanova Aizhan, Aukenov Nurlan, Nurtazina Alma, Massabayeva Meruyert, Babenko Dmitriy, Adiyeva Madina, Shaimardonov Nurlan
Department of Propaedeutic of Internal Diseases, Semey Medical University, Semey, East Kazakhstan Region F17G0D3, Kazakhstan.
Department of Health and Human Resources, Ministry of Health of the Republic of Kazakhstan, Nur-Sultan, East Kazakhstan Region Z05K5K8, Kazakhstan.
Biomed Rep. 2020 Nov;13(5):35. doi: 10.3892/br.2020.1342. Epub 2020 Aug 7.
Hyperinsulinism and insulin resistance are closely associated with several common diseases including type 2 of diabetes, cardiovascular diseases, and metabolic syndrome. The present study aimed to determine the association between hyperinsulinism, insulin resistance and the polymorphism of genes, including (), (), () and (), in the Kazakh population. The design of the current research was a case-control study, involving 460 subjects (age range, 18-65 years). For every subject, plasma glucose, insulin, total cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, apolipoprotein B and apolipoprotein A1 were examined. Moreover, reverse transcription-quantitative PCR was conducted to detect the polymorphism genes and . Hyperinsulinism was considered when the insulin level was elevated >24.9 IU/ml. The homeostasis model assessment insulin resistance (HOMA-IR) was used to evaluate insulin resistance. The subjects were divided into hyperinsulinism (17 men and 24 women) and normal level insulin (214 men and 205 women) groups, which were also split into insulin resistance group (HOMA-IR >2.7; 80 men and 105 women) and those without insulin resistance group (151 men and 124 women). The results suggested that () allele G was associated with a lower risk of hyperinsulinism (P=0.037). Furthermore, polymorphisms of genes and were not associated with hyperinsulinism and insulin resistance in the Kazakh population. No interaction was identified between and . Therefore, the results indicated that haplotype combinations were not associated with insulin resistance.
高胰岛素血症和胰岛素抵抗与包括2型糖尿病、心血管疾病和代谢综合征在内的几种常见疾病密切相关。本研究旨在确定哈萨克族人群中高胰岛素血症、胰岛素抵抗与包括（）、（）、（）和（）在内的基因多态性之间的关联。当前研究的设计为病例对照研究，涉及460名受试者（年龄范围为18 - 65岁）。对每位受试者检测血浆葡萄糖、胰岛素、总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白、载脂蛋白B和载脂蛋白A1。此外，进行逆转录定量PCR以检测多态性基因和。当胰岛素水平升高>24.9 IU/ml时考虑为高胰岛素血症。采用稳态模型评估胰岛素抵抗（HOMA - IR）来评估胰岛素抵抗。受试者被分为高胰岛素血症组（17名男性和24名女性）和胰岛素正常水平组（214名男性和205名女性），这两组又进一步分为胰岛素抵抗组（HOMA - IR>2.7；80名男性和105名女性）和无胰岛素抵抗组（151名男性和124名女性）。结果表明（）基因的G等位基因与较低的高胰岛素血症风险相关（P = 0.037）。此外，在哈萨克族人群中，基因和的多态性与高胰岛素血症和胰岛素抵抗无关。未发现与之间存在相互作用。因此，结果表明单倍型组合与胰岛素抵抗无关。
