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长链非编码RNA PCNA-AS1的高表达通过上调CCND1促进非小细胞肺癌细胞增殖和致癌活性。

High Expression of Long Noncoding RNA PCNA-AS1 Promotes Non-Small-Cell Lung Cancer Cell Proliferation and Oncogenic Activity via Upregulating CCND1.

作者信息

Wu Chuanyong, Zhu Xiao-Ting, Xia Lei, Wang Lin, Yu Wenjun, Guo Qiaomei, Zhao Mingna, Lou Jiatao

机构信息

Department of Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China.

Department of Laboratory Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

出版信息

J Cancer. 2020 Jan 29;11(7):1959-1967. doi: 10.7150/jca.39087. eCollection 2020.

DOI:10.7150/jca.39087
PMID:32194807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7052854/
Abstract

Accumulating evidences showed that aberrantly expressed long noncoding RNAs (lncRNAs) have critical roles in many cancers. However, the expression and roles of a poorly studied lncRNA PCNA-AS1 in non-small-cell lung cancer (NSCLC) remain unknown. In this study, we investigated the expression, clinical significance, biological roles, and functional mechanism of PCNA-AS1 in NSCLC. Our results showed that PCNA-AS1 was upregulated in NSCLC tissues and cell lines, and correlated with TNM stages. Functional experiments showed that overexpression of PCNA-AS1 promoted NSCLC cell proliferation and cell cycle progression. Depletion of PCNA-AS1 inhibited NSCLC cell proliferation and cell cycle progression, and also inhibited NSCLC tumor growth . Mechanistically, we found that PCNA-AS1 upregulated CCND1 expression. The expression of PCNA-AS1 was positively correlated with that of CCND1 in NSCLC tissues. Moreover, depletion of CCND1 abrogated the oncogenic roles of PCNA-AS1 in NSCLC. In conclusion, highly expressed PCNA-AS1 promotes NSCLC cell proliferation and oncogenic activity via upregulating CCND1. Our results imply that PCNA-AS1 might serve as a therapeutic target for NSCLC.

摘要

越来越多的证据表明，异常表达的长链非编码RNA（lncRNA）在许多癌症中发挥着关键作用。然而，研究较少的lncRNA PCNA-AS1在非小细胞肺癌（NSCLC）中的表达和作用仍不清楚。在本研究中，我们调查了PCNA-AS1在NSCLC中的表达、临床意义、生物学作用和功能机制。我们的结果表明，PCNA-AS1在NSCLC组织和细胞系中上调，并与TNM分期相关。功能实验表明，PCNA-AS1的过表达促进了NSCLC细胞的增殖和细胞周期进程。PCNA-AS1的缺失抑制了NSCLC细胞的增殖和细胞周期进程，也抑制了NSCLC肿瘤的生长。机制上，我们发现PCNA-AS1上调了CCND1的表达。在NSCLC组织中，PCNA-AS1的表达与CCND1的表达呈正相关。此外，CCND1的缺失消除了PCNA-AS1在NSCLC中的致癌作用。总之，高表达的PCNA-AS1通过上调CCND1促进NSCLC细胞增殖和致癌活性。我们的结果表明，PCNA-AS1可能成为NSCLC的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de07/7052854/9a3d7939f311/jcav11p1959g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de07/7052854/2c5faf91f98d/jcav11p1959g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de07/7052854/677bf3c5f16c/jcav11p1959g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de07/7052854/cd038991502d/jcav11p1959g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de07/7052854/9a3d7939f311/jcav11p1959g007.jpg

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