Schvartzman Claudia, Fresia Pablo, Murchio Sara, Mujica María Valentina, Dalla-Rizza Marco
Unidad de Biotecnología, Instituto Nacional de Investigación Agropecuaria, Canelones, Uruguay.
Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Montevideo, Uruguay.
Front Plant Sci. 2022 Apr 1;13:804839. doi: 10.3389/fpls.2022.804839. eCollection 2022.
Red-banded stink bug () has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in to develop new pest-control strategies. For this, we assembled and annotated a transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting . A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of gene of 84% 72 h post-injection. In addition, and genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies.
红带蝽（）被认为是对大豆作物危害最大的蝽象，在低密度种群情况下会导致种子受损、发芽率降低以及叶片留存。近年来，RNA干扰（RNAi）这种保守的真核生物沉默机制已被探索用于开发物种选择性农药。在这项工作中，我们评估了红带蝽的RNAi以制定新的害虫控制策略。为此，我们从所有发育阶段的样本中组装并注释了一个转录组。对该转录组的分析导致鉴定出56个与沉默过程相关的基因，包括小干扰RNA（siRNA）、微小RNA（miRNA）和PIWI相互作用RNA（piRNA）途径。为了评估RNAi机制的功能，给红带蝽成虫注射了每毫克体重28纳克的靶向的双链RNA（dsRNA）。分别在7天和14天后观察到死亡率为35%和51.6%，注射后72小时基因下调了84%。此外，核心RNAi蛋白和基因在注射后48小时上调了1.8倍。这些发现首次表明RNAi在红带蝽中具有功能，并且必需基因的沉默对成虫活力有显著影响。综上所述，此处报道的工作表明RNAi可能是开发红带蝽控制策略的一种有趣方法。
