RNAi in (Hemiptera: Pentatomidae): Transcriptome Assembly for the Development of Pest Control Strategies.

Red-banded stink bug () has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in to develop new pest-control strategies. For this, we assembled and annotated a transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting . A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of gene of 84% 72 h post-injection. In addition, and genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies.