Oduola A M, Weatherly N F, Bowdre J H, Desjardins R E
Department of Parasitology and Laboratory Practice, School of Public Health, University of North Carolina, Chapel Hill 27514.
Exp Parasitol. 1988 Jun;66(1):86-95. doi: 10.1016/0014-4894(88)90053-7.
Natural isolates of Plasmodium falciparum represent a genetically heterogeneous population of parasites. To obtain stable strains of the parasites for long term experiments, a rapid and definitive method of cloning was developed using micropipets and a micromanipulator. Homogeneous parasite clones prepared by this technique were characterized and compared with the parent isolates during 4 years of continuous culture. The process of phenotypic dominance and selection of drug resistance which occur in nature was also simulated in vitro by evaluating population dynamics of two cocultured isolates of P. falciparum.
恶性疟原虫的天然分离株代表了寄生虫的遗传异质群体。为了获得用于长期实验的稳定寄生虫株，开发了一种使用微量移液器和显微操作器的快速且确定的克隆方法。通过该技术制备的同质寄生虫克隆在连续培养的4年中进行了表征，并与亲本分离株进行了比较。通过评估两种共培养的恶性疟原虫分离株的群体动态，还在体外模拟了自然界中发生的表型优势和耐药性选择过程。
