体外修复大肠杆菌单碱基对颠换错配：某些A/G错配的校正独立于dam甲基化和宿主mutHLS基因功能。
Repair of single base-pair transversion mismatches of Escherichia coli in vitro: correction of certain A/G mismatches is independent of dam methylation and host mutHLS gene functions.
作者信息
Lu A L, Chang D Y
机构信息
Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201.
出版信息
Genetics. 1988 Apr;118(4):593-600. doi: 10.1093/genetics/118.4.593.
Abstract
Six different base-pair transversion mismatches are repaired with different efficiencies in an in vitro mismatch repair system. In particular, the T/T and C/C mismatches appear to be less efficiently repaired than the A/A and G/G mismatches. Four A/G and four C/T mismatches at different positions are repaired to different extents. One of the A/G mismatches is repaired equally efficiently when DNA heteroduplexes are fully methylated or hemi-methylated at the d(GATC) sequences. This type of mismatch repair appears to be unidirectional with A to C conversion by acting at A/G mispairs to restore the C/G pairs. This methylation-independent correction is not controlled by the mutH, mutL, mutS, uvrE, uvrB, phr, recA, recF, and recJ gene products. The independence of the transversion mismatch repair of these genes and methylation distinguishes this from the known mismatch repair pathways.
摘要
在体外错配修复系统中，六种不同的碱基对颠换错配以不同的效率被修复。具体而言，T/T和C/C错配的修复效率似乎低于A/A和G/G错配。不同位置的四个A/G和四个C/T错配被修复的程度不同。当DNA异源双链在d(GATC)序列处完全甲基化或半甲基化时，其中一个A/G错配的修复效率相同。这种错配修复似乎是单向的，通过作用于A/G错配将A转换为C以恢复C/G对。这种不依赖甲基化的校正不受mutH、mutL、mutS、uvrE、uvrB、phr、recA、recF和recJ基因产物的控制。这些基因和甲基化在颠换错配修复中的独立性使其有别于已知的错配修复途径。
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