Yada R Y, Jackman R L, Nakai S
Department of Food Science, University of Guelph, Ontario, Canada.
Int J Pept Protein Res. 1988 Jan;31(1):98-108. doi: 10.1111/j.1399-3011.1988.tb00011.x.
The rapid increase in sequence data in combination with a greater understanding of the forces regulating protein structure has been the impetus for an upsurge in the development of theoretical prediction methods. These methods have afforded protein chemists the ability to identify and quantify the various secondary structures along the protein chain. Concurrently, various physico-chemical techniques have been developed such as nuclear Overhauser enhancement n.m.r. and laser Raman spectroscopy. In addition, traditional methods such as infrared and circular dichroism spectroscopy have been refined. Although both predictive and physico-chemical techniques are limited in the types of secondary structure they are capable of determining, they have provided valuable information with regards to protein folding and topology in the absence of X-ray data, and have formed the basis for the development of improved methods for secondary structure determination. This paper reviews some of the predictive and physico-chemical methods presently used to determine protein secondary structure.
序列数据的迅速增加，再加上对调节蛋白质结构的作用力有了更深入的了解，推动了理论预测方法的蓬勃发展。这些方法使蛋白质化学家能够识别和量化蛋白质链上的各种二级结构。同时，还开发了各种物理化学技术，如核Overhauser增强核磁共振和激光拉曼光谱。此外，红外和圆二色光谱等传统方法也得到了改进。尽管预测技术和物理化学技术在能够确定的二级结构类型方面都有局限性，但在没有X射线数据的情况下，它们提供了有关蛋白质折叠和拓扑结构的有价值信息，并为开发改进的二级结构测定方法奠定了基础。本文综述了目前用于确定蛋白质二级结构的一些预测方法和物理化学方法。
