Kao K J, Scornik J C, Riley W J, McQueen C F
Department of Pathology, College of Medicine, University of Florida, Gainesville 32610.
Hum Immunol. 1988 Feb;21(2):115-24. doi: 10.1016/0198-8859(88)90086-9.
To understand the relationship between HLA phenotype and plasma or platelet HLA better, concentrations of plasma and platelet HLA were measured in 215 individuals of known HLA phenotypes. Precise quantitation of HLA antigens was achieved by means of an enzyme-linked immunoassay using the W6/32 monoclonal antibody and purified HLA molecules. The mean plasma and platelet HLA concentrations were 2.04 +/- 1.67 micrograms/ml (+/- SD, n = 215) and 11.28 +/- 4.65 fg/cell (+/- SD, n = 213), respectively. Statistical analysis of associations between HLA phenotypes and plasma HLA revealed that the mean plasma HLA concentration of individuals with HLA-A23 or HLA-A24 was 1.4 (p less than 0.002) or 1.9 (p less than 0.001) times higher than those without these two HLA antigens. Furthermore, the mean plasma HLA concentration of individuals who have HLA-A26 was 25% less than those without HLA-A26 (p less than 0.05). In contrast, the only association between HLA phenotypes and HLA concentrations of platelets was observed in HLA-B7-positive individuals. The mean platelet HLA concentration of HLA-B7 individuals was 27% higher than those without HLA-B7 (p less than 0.005). This finding is in accordance with previous observations made on red blood cells. The results indicate that the HLA concentrations in plasma are regulated, at least in part, by genetic factors that are different from those regulating platelet HLA.
为了更好地理解HLA表型与血浆或血小板HLA之间的关系，对215名已知HLA表型的个体进行了血浆和血小板HLA浓度的测量。通过使用W6/32单克隆抗体和纯化的HLA分子的酶联免疫测定法实现了HLA抗原的精确定量。血浆和血小板HLA的平均浓度分别为2.04±1.67微克/毫升（±标准差，n = 215）和11.28±4.65飞克/细胞（±标准差，n = 213）。对HLA表型与血浆HLA之间关联的统计分析表明，具有HLA-A23或HLA-A24的个体的平均血浆HLA浓度比没有这两种HLA抗原的个体高1.4倍（p<0.002）或1.9倍（p<0.001）。此外，具有HLA-A26的个体的平均血浆HLA浓度比没有HLA-A26的个体低25%（p<0.05）。相比之下，HLA表型与血小板HLA浓度之间的唯一关联在HLA-B7阳性个体中观察到。HLA-B7个体的平均血小板HLA浓度比没有HLA-B7的个体高27%（p<0.005）。这一发现与先前对红细胞的观察结果一致。结果表明，血浆中的HLA浓度至少部分受与调节血小板HLA的遗传因素不同的遗传因素调节。
