Castiglione Roberto, Calogero Aldo E, Vicari Enzo, Calabrini Giovanna, Cosentino Anna, D'Agati Placido, Fraggetta Filippo, Salemi Michele
Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
Pathology Unit, Gravina Hospital, Caltagirone, Italy.
Case Rep Gastrointest Med. 2020 Jun 29;2020:2717150. doi: 10.1155/2020/2717150. eCollection 2020.
Pancreatic cancer is a most frequent cancer in Europe, and the majority of cases of cancer of the pancreas are diagnosed above the age of 65. Radical surgery is the first curative treatment of pancreatic cancer, and alternative or combined therapeutic options, in particular, consist of adjuvant or neoadjuvant chemotherapy, with or without radiotherapy. Many factors, including diet and genetics, have been implicated in the development of cancer of the pancreas. Poly (ADP-ribose) polymerase 1 (PARP-1) protein is required for translocation of the apoptosis-inducing factor (AIF) from the mitochondria to the nucleus. It is involved in programmed cell death processes. Different PARP-1 gene expression proteins have been observed in various tumors such as lung, ovarian, endometrial, skin, and glioblastoma. We evaluated the expression of PARP-1 protein in pancreatic adenocarcinoma and normal pancreas tissues by immunohistochemistry. Protein PARP-1 in the nucleus was found in all samples (normal pancreas and pancreatic adenocarcinoma tissues). No cytoplasmic staining was observed in any sample. PARP-1-positive cells resulted higher in the normal pancreas compared with the pancreas with adenocarcinoma. PARP-1 overexpression in prostate cancer tissue compared with normal prostate suggests a greater activity of PARP-1 in these tumors. These findings suggest that PARP-1 expression in prostate cancer is an attempt to trigger apoptosis in this type of tumor, similarl to that reported in other cancers. This finding suggests that PARP-1-mediated cell death pathways are inhibited in this cancer.
胰腺癌是欧洲最常见的癌症之一，大多数胰腺癌病例在65岁以上被诊断出来。根治性手术是胰腺癌的首要治愈性治疗方法，其他治疗选择或联合治疗方案尤其包括辅助或新辅助化疗，可联合或不联合放疗。许多因素，包括饮食和基因，都与胰腺癌的发生有关。聚（ADP-核糖）聚合酶1（PARP-1）蛋白是凋亡诱导因子（AIF）从线粒体转运至细胞核所必需的。它参与程序性细胞死亡过程。在各种肿瘤如肺癌、卵巢癌、子宫内膜癌、皮肤癌和胶质母细胞瘤中已观察到不同的PARP-1基因表达蛋白。我们通过免疫组织化学评估了PARP-1蛋白在胰腺腺癌和正常胰腺组织中的表达。在所有样本（正常胰腺和胰腺腺癌组织）中均发现细胞核中的PARP-1蛋白。在任何样本中均未观察到细胞质染色。与胰腺腺癌相比，正常胰腺中PARP-1阳性细胞更多。与正常前列腺相比，前列腺癌组织中PARP-1过表达表明PARP-1在这些肿瘤中的活性更高。这些发现表明，前列腺癌中PARP-1的表达是试图在这类肿瘤中触发凋亡，类似于在其他癌症中所报道的情况。这一发现表明PARP-1介导的细胞死亡途径在这种癌症中受到抑制。
