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基于CRISPR/Cas9转录调控系统的细胞重编程

Cell Reprogramming With CRISPR/Cas9 Based Transcriptional Regulation Systems.

作者信息

Shakirova Ksenia M, Ovchinnikova Viktoriia Y, Dashinimaev Erdem B

机构信息

Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.

Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.

出版信息

Front Bioeng Biotechnol. 2020 Jul 28;8:882. doi: 10.3389/fbioe.2020.00882. eCollection 2020.

DOI:10.3389/fbioe.2020.00882
PMID:32850737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7399070/
Abstract

The speed of reprogramming technologies evolution is rising dramatically in modern science. Both the scientific community and health workers depend on such developments due to the lack of safe autogenic cells and tissues for regenerative medicine, genome editing tools and reliable screening techniques. To perform experiments efficiently and to propel the fundamental science it is important to keep up with novel modifications and techniques that are being discovered almost weekly. One of them is CRISPR/Cas9 based genome and transcriptome editing. The aim of this article is to summarize currently existing CRISPR/Cas9 applications for cell reprogramming, mainly, to compare them with other non-CRISPR approaches and to highlight future perspectives and opportunities.

摘要

在现代科学中，重编程技术的发展速度正在急剧上升。由于再生医学缺乏安全的自体细胞和组织、基因组编辑工具以及可靠的筛选技术，科学界和医疗工作者都依赖于此类发展。为了高效地进行实验并推动基础科学发展，跟上几乎每周都在发现的新修饰和新技术很重要。其中之一就是基于CRISPR/Cas9的基因组和转录组编辑。本文的目的是总结目前现有的CRISPR/Cas9在细胞重编程中的应用，主要是将它们与其他非CRISPR方法进行比较，并突出未来的前景和机遇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/c554094bd6bd/fbioe-08-00882-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/58b2283c951b/fbioe-08-00882-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/ecde2c077170/fbioe-08-00882-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/0f4902f94616/fbioe-08-00882-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/c554094bd6bd/fbioe-08-00882-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/58b2283c951b/fbioe-08-00882-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/ecde2c077170/fbioe-08-00882-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/0f4902f94616/fbioe-08-00882-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69b/7399070/c554094bd6bd/fbioe-08-00882-g004.jpg

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