Ruzauskas M, Couto N, Pavilonis A, Klimiene I, Siugzdiniene R, Virgailis M, Vaskeviciute L, Anskiene L, Pomba C
Pol J Vet Sci. 2016;19(1):7-14. doi: 10.1515/pjvs-2016-0002.
The aim of this study was to characterize Staphylococcus pseudintermedius for its antimicrobial resistance and virulence factors with a special focus on methicillin-resistant (MRSP) strains isolated from sick dogs in Lithuania. Clinically sick adult dogs suffering from infections (n=214) and bitches with reproductive disorders (n=36) from kennels were selected for the study. Samples (n=192) from the 250 tested (76.8%) dogs were positive for Staphylococcus spp. Molecular profiling using the species-specific nuc gene identified 51 isolates as S. pseudintermedius (26.6% from a total number of isolated staphylococci) of which 15 isolates were identified as MRSP. Ten MRSP isolates were isolated from bitches with reproductive disorders from two large breeding kennels. Data on susceptibility of S. pseudintermedius to different antimicrobials revealed that all isolates were susceptible to vancomycin, daptomycin and linezolid. Two isolates (3.9%) were resistant to rifampicin. A high resistance was seen towards penicillin G (94.1%), tetracycline (64.7%) and macrolides (68.7%). Resistance to fluoroquinolones ranged from 25.5% (gatifloxacin) to 31.4% (ciprofloxacin). The most prevalent genes encoding resistance included blaZ, aac(6')-Ie-aph(2'')-Ia, mecA, and tet(M). The Luk-I gene encoding a leukotoxin was detected in 29% of the isolates, whereas the siet gene encoding exfoliative toxin was detected in 69% of the S. pseudintermedius isolates. This report of MRSP in companion animals represents a major challenge for veterinarians in terms of antibiotic therapy and is a concern for both animal and public health.
本研究的目的是对中间型葡萄球菌的耐药性和毒力因子进行表征，特别关注从立陶宛患病犬只中分离出的耐甲氧西林（MRSP）菌株。选择了来自犬舍的患有感染的临床患病成年犬（n = 214）和患有生殖系统疾病的母犬（n = 36）进行研究。在250只检测犬中，有192只（76.8%）的样本葡萄球菌属呈阳性。使用物种特异性nuc基因进行分子谱分析，鉴定出51株为中间型葡萄球菌（占分离出的葡萄球菌总数的26.6%），其中15株被鉴定为MRSP。从两个大型繁殖犬舍患有生殖系统疾病的母犬中分离出10株MRSP菌株。中间型葡萄球菌对不同抗菌药物的敏感性数据显示，所有分离株对万古霉素、达托霉素和利奈唑胺敏感。两株分离株（3.9%）对利福平耐药。对青霉素G（94.1%）、四环素（64.7%）和大环内酯类（68.7%）耐药性较高。对氟喹诺酮类的耐药性范围为25.5%（加替沙星）至31.4%（环丙沙星）。编码耐药性的最常见基因包括blaZ、aac(6')-Ie-aph(2'')-Ia、mecA和tet(M)。在29%的分离株中检测到编码白细胞毒素的Luk-I基因，而在69%的中间型葡萄球菌分离株中检测到编码剥脱毒素的siet基因。伴侣动物中MRSP的这一报告在抗生素治疗方面对兽医构成了重大挑战，并且对动物和公共卫生都构成了担忧。
