Department of Pathology, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250014, China.
Department of Pathology, Tai'an Central Hospital, Tai'an 271000, China.
Biomed Res Int. 2020 Aug 10;2020:9312149. doi: 10.1155/2020/9312149. eCollection 2020.
The purpose of this study was to investigate the effect of metformin or the combination of metformin and 5-FU on the growth and metastasis of colorectal cancer (CRC). For the experiments, HCT 116 and SW1463 cell lines were treated with metformin or the combination of metformin and 5-FU. Cell proliferation and invasion were analyzed by CCK-8, colony formation, and transwell assay, respectively. For the experiments, the CRC xenograft nude mice model was used to observe the effects of metformin or combined with 5-FU on tumor growth and metastasis. Metformin significantly inhibited the proliferation and invasion of HCT116 and SW1463 cells , which showed synergetic effects to 5-FU. In CRC xenograft nude mice, metformin alone and metformin combined with 5-FU treatment significantly inhibited tumor cell proliferation and tumor metastasis. In summary, metformin played an inhibitory role in the proliferation and metastasis of CRC and had a synergistic effect with 5-FU. Metformin may be a potentially effective anti-metastatic drug or an anticancer adjuvant agent for treating CRC.
本研究旨在探讨二甲双胍或二甲双胍联合 5-FU 对结直肠癌（CRC）生长和转移的影响。在实验中，用二甲双胍或二甲双胍联合 5-FU 处理 HCT116 和 SW1463 细胞系。分别通过 CCK-8、集落形成和 Transwell 分析检测细胞增殖和侵袭。在实验中，使用 CRC 异种移植裸鼠模型观察二甲双胍或联合 5-FU 对肿瘤生长和转移的影响。二甲双胍显著抑制 HCT116 和 SW1463 细胞的增殖和侵袭，与 5-FU 表现出协同作用。在 CRC 异种移植裸鼠中，二甲双胍单独和联合 5-FU 治疗均显著抑制肿瘤细胞增殖和肿瘤转移。综上所述，二甲双胍在 CRC 的增殖和转移中发挥抑制作用，与 5-FU 具有协同作用。二甲双胍可能是一种有潜力的抗转移药物或治疗 CRC 的抗癌辅助药物。
