Ki67指数与循环肿瘤细胞在结直肠癌诊断中的独立相关性

Independent Correlation Between Ki67 Index and Circulating Tumor Cells in the Diagnosis of Colorectal Cancer.

作者信息

Yang Yingchi, Li Jun, Jin Lan, Wang Dong, Zhang Jinghui, Wang Jin, Zhao Xiaomu, Wu Guocong, Yao Hongwei, Zhang Zhongtao

机构信息

Department of General Surgery, Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center for Digestive Diseases, Beijing Friendship Hospital, Capital MedicalUniversity, Xi-Cheng District, Beijing, P.R. China.

出版信息

Anticancer Res. 2017 Aug;37(8):4693-4700. doi: 10.21873/anticanres.11874.

DOI:10.21873/anticanres.11874

PMID:28739773

Abstract

BACKGROUND

The Ki67 index is a biomarker of proliferation, while the circulating tumor cell (CTC) count acts as a metastasis-related biomarker. In this study, we analyzed the potential value of CTC count and Ki67 index in diagnosis of colorectal cancer (CRC).

MATERIALS AND METHODS

A total of 105 patients with CRC undergoing surgery were included in the study. Isolation and identification of CTCs were performed by negative enrichment and immuno-fluorescence in situ hybridization, respectively. Expression of Ki67 was assessed by immunohistochemistry.

RESULTS

Patients with CTC count ≥2 were defined as CTC-positive. Ki67 index ≥50% was regarded as highly proliferative. Overall, 71 cases were CTC-positive, while 82 cases displayed a high Ki67 index. CTC count and Ki67 index had no correlation with tumor size, tumor site, age, gender and TNM stages of the patients. CTC count was correlated with tumor size (p=0.018) and Ki67 index with level of differentiation (p<0.001). However, there was no relationship between CTC count and Ki67 index (p=0.198).

CONCLUSION

Our results suggest that CTCs can act as a potent metastasis-related biomarker for the diagnosis of CRC, independently of the Ki67 index.

摘要

背景

Ki67指数是一种增殖生物标志物，而循环肿瘤细胞（CTC）计数则作为一种与转移相关的生物标志物。在本研究中，我们分析了CTC计数和Ki67指数在结直肠癌（CRC）诊断中的潜在价值。

材料与方法

本研究共纳入105例接受手术的CRC患者。分别通过阴性富集和免疫荧光原位杂交进行CTC的分离和鉴定。通过免疫组织化学评估Ki67的表达。

结果

CTC计数≥2的患者被定义为CTC阳性。Ki67指数≥50%被视为高增殖性。总体而言，71例患者CTC阳性，82例患者Ki67指数高。CTC计数和Ki67指数与患者的肿瘤大小、肿瘤部位、年龄、性别和TNM分期均无相关性。CTC计数与肿瘤大小相关（p = 0.018），Ki67指数与分化程度相关（p < 0.001）。然而，CTC计数与Ki67指数之间无相关性（p = 0.198）。