赋予酿酒酵母中杂合缺失高减数分裂后分离频率的DNA序列与真核生物重组热点相关序列有关。
A DNA sequence conferring high postmeiotic segregation frequency to heterozygous deletions in Saccharomyces cerevisiae is related to sequences associated with eucaryotic recombination hotspots.
作者信息
White J H, DiMartino J F, Anderson R W, Lusnak K, Hilbert D, Fogel S
机构信息
Department of Genetics, University of California, Berkeley 94720.
出版信息
Mol Cell Biol. 1988 Mar;8(3):1253-8. doi: 10.1128/mcb.8.3.1253-1258.1988.
Abstract
The meiotic behavior of two graded series of deletion mutations in the ADE8 gene in Saccharomyces cerevisiae was analyzed to investigate the molecular basis of meiotic recombination. Postmeiotic segregation (PMS) was observed for a subset of the deletion heterozygosities, including deletions of 38 to 93 base pairs. There was no clear relationship between deletion length and PMS frequency. A common sequence characterized the novel joint region in the alleles which displayed PMS. This sequence is related to repeated sequences recently identified in association with recombination hotspots in the human and mouse genomes. We propose that these particular deletion heterozygosities escape heteroduplex DNA repair because of fortuitous homology to a binding site for a protein.
摘要
为了研究减数分裂重组的分子基础，对酿酒酵母中ADE8基因的两个分级系列缺失突变的减数分裂行为进行了分析。在一部分缺失杂合子中观察到了减数分裂后分离（PMS），包括38至93个碱基对的缺失。缺失长度与PMS频率之间没有明确的关系。在显示PMS的等位基因的新连接区域有一个共同序列。该序列与最近在人类和小鼠基因组中与重组热点相关联而鉴定出的重复序列有关。我们提出，这些特定的缺失杂合子由于与一种蛋白质的结合位点偶然同源而逃避了异源双链DNA修复。
相似文献
1
A DNA sequence conferring high postmeiotic segregation frequency to heterozygous deletions in Saccharomyces cerevisiae is related to sequences associated with eucaryotic recombination hotspots.赋予酿酒酵母中杂合缺失高减数分裂后分离频率的DNA序列与真核生物重组热点相关序列有关。
Mol Cell Biol. 1988 Mar;8(3):1253-8. doi: 10.1128/mcb.8.3.1253-1258.1988.
2
Repair of specific base pair mismatches formed during meiotic recombination in the yeast Saccharomyces cerevisiae.酿酒酵母减数分裂重组过程中形成的特定碱基对错配的修复。
Mol Cell Biol. 1991 Feb;11(2):737-45. doi: 10.1128/mcb.11.2.737-745.1991.
3
Poorly repaired mismatches in heteroduplex DNA are hyper-recombinagenic in Saccharomyces cerevisiae.在酿酒酵母中，异源双链DNA修复不佳的错配具有高度重组活性。
Genetics. 1996 Feb;142(2):407-16. doi: 10.1093/genetics/142.2.407.
4
Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae.协同缺失和倒位是由酿酒酵母中δ序列之间的有丝分裂重组引起的。
Mol Cell Biol. 1987 Mar;7(3):1198-207. doi: 10.1128/mcb.7.3.1198-1207.1987.
5
Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate.酵母中错配特异性减数分裂后分离频率表明存在异源双链体重组中间体。
Nature. 1985;315(6017):350-2. doi: 10.1038/315350a0.
6
A test of the CoHR motif associated with meiotic double-strand breaks in Saccharomyces cerevisiae.一项关于与酿酒酵母减数分裂双链断裂相关的CoHR基序的测试。
EMBO Rep. 2004 Jan;5(1):41-6. doi: 10.1038/sj.embor.7400050.
7
Mutations in POL1 increase the mitotic instability of tandem inverted repeats in Saccharomyces cerevisiae.POL1基因的突变会增加酿酒酵母中串联反向重复序列的有丝分裂不稳定性。
Genetics. 1993 May;134(1):43-56. doi: 10.1093/genetics/134.1.43.
8
HPR1, a novel yeast gene that prevents intrachromosomal excision recombination, shows carboxy-terminal homology to the Saccharomyces cerevisiae TOP1 gene.HPR1是一种新型酵母基因，可防止染色体内切除重组，其羧基末端与酿酒酵母TOP1基因具有同源性。
Mol Cell Biol. 1990 Apr;10(4):1439-51. doi: 10.1128/mcb.10.4.1439-1451.1990.
9
Replication slippage between distant short repeats in Saccharomyces cerevisiae depends on the direction of replication and the RAD50 and RAD52 genes.酿酒酵母中远距离短重复序列之间的复制滑移取决于复制方向以及RAD50和RAD52基因。
Mol Cell Biol. 1995 Oct;15(10):5607-17. doi: 10.1128/MCB.15.10.5607.
10
Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae.酿酒酵母中HIS4位点基因转换梯度的分析。
Genetics. 1992 Sep;132(1):113-23. doi: 10.1093/genetics/132.1.113.
引用本文的文献
1
Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae.酿酒酵母中氨基酸、核苷酸和磷酸盐代谢的调节。
Genetics. 2012 Mar;190(3):885-929. doi: 10.1534/genetics.111.133306.
2
The large loop repair and mismatch repair pathways of Saccharomyces cerevisiae act on distinct substrates during meiosis.酿酒酵母的大环状修复和错配修复途径在减数分裂过程中作用于不同的底物。
Genetics. 2005 Jul;170(3):1033-43. doi: 10.1534/genetics.104.033670. Epub 2005 May 6.
3
Efficient repair of large DNA loops in Saccharomyces cerevisiae.酿酒酵母中大型DNA环的高效修复。
Nucleic Acids Res. 2001 Oct 15;29(20):4134-43. doi: 10.1093/nar/29.20.4134.
4
Microsatellite evolution: polarity of substitutions within repeats and neutrality of flanking sequences.微卫星进化：重复序列内替换的极性及侧翼序列的中性
Proc Biol Sci. 1999 Apr 22;266(1421):825-33. doi: 10.1098/rspb.1999.0712.
5
A 140-bp-long palindromic sequence induces double-strand breaks during meiosis in the yeast Saccharomyces cerevisiae.一段140个碱基对长的回文序列在酿酒酵母减数分裂过程中诱导双链断裂。
Genetics. 1997 Jul;146(3):835-47. doi: 10.1093/genetics/146.3.835.
6
Instability of CAG and CTG trinucleotide repeats in Saccharomyces cerevisiae.酿酒酵母中CAG和CTG三核苷酸重复序列的不稳定性。
Mol Cell Biol. 1997 Jun;17(6):3382-7. doi: 10.1128/MCB.17.6.3382.
7
Poorly repaired mismatches in heteroduplex DNA are hyper-recombinagenic in Saccharomyces cerevisiae.在酿酒酵母中，异源双链DNA修复不佳的错配具有高度重组活性。
Genetics. 1996 Feb;142(2):407-16. doi: 10.1093/genetics/142.2.407.
8
The prevention of repeat-associated deletions in Saccharomyces cerevisiae by mismatch repair depends on size and origin of deletions.酿酒酵母中错配修复对重复相关缺失的预防取决于缺失的大小和起源。
Genetics. 1996 Aug;143(4):1579-87. doi: 10.1093/genetics/143.4.1579.
9
Recognition of DNA insertion/deletion mismatches by an activity in Saccharomyces cerevisiae.酿酒酵母中一种活性对DNA插入/缺失错配的识别
Nucleic Acids Res. 1996 Feb 15;24(4):721-9. doi: 10.1093/nar/24.4.721.
10
Translation of the yeast transcriptional activator GCN4 is stimulated by purine limitation: implications for activation of the protein kinase GCN2.酵母转录激活因子GCN4的翻译受嘌呤限制的刺激：对蛋白激酶GCN2激活的影响。
Mol Cell Biol. 1993 Aug;13(8):5099-111. doi: 10.1128/mcb.13.8.5099-5111.1993.
本文引用的文献
1
Structure of chi hotspots of generalized recombination.广义重组的chi热点结构
Cell. 1981 May;24(2):429-36. doi: 10.1016/0092-8674(81)90333-0.
2
Physical monitoring of meiotic recombination in Saccharomyces cerevisiae.酿酒酵母减数分裂重组的物理监测
Cold Spring Harb Symp Quant Biol. 1984;49:67-76. doi: 10.1101/sqb.1984.049.01.010.
3
A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast.一种用于聚乙二醇诱导细菌和酵母遗传转化的通用方法。
Gene. 1983 Nov;25(2-3):333-41. doi: 10.1016/0378-1119(83)90238-x.
4
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
5
A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
6
Postmeiotic segregation in Saccharomyces.酿酒酵母中的减数分裂后分离
Mol Gen Genet. 1971;111(3):297-9. doi: 10.1007/BF00433113.
7
Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate.酵母中错配特异性减数分裂后分离频率表明存在异源双链体重组中间体。
Nature. 1985;315(6017):350-2. doi: 10.1038/315350a0.
8
Gene organization and recombinational hotspots in the murine major histocompatibility complex.小鼠主要组织相容性复合体中的基因组织与重组热点
Cell. 1986 Mar 28;44(6):895-904. doi: 10.1016/0092-8674(86)90012-7.
9
Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex.小鼠主要组织相容性复合体中重组热点的分子分析。
Science. 1986 Oct 10;234(4773):173-9. doi: 10.1126/science.3018929.
10
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
Zotero 插件