Application of new techniques for the detection of carcinogen adducts to human population monitoring.

Several techniques have recently been developed for the detection and quantitation of carcinogen-DNA or -protein adducts without the requirement for radioactive carcinogens. These assays can be used to detect adducts in animals or cultured cells exposed to test compounds or in humans exposed to environmental carcinogens. Immunologic, 32P-postlabeling and fluorescence techniques, used on human samples for DNA adduct measurement, are reviewed here. Methods for the detection of carcinogen-protein adducts on human samples are also summarized.