van de Vijver M J, Mooi W J, Wisman P, Peterse J L, Nusse R
Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam.
Oncogene. 1988 Feb;2(2):175-8.
Amplification of the neu (or c-erbB-2 or HER) oncogene is relatively frequent in human breast carcinomas. We have raised a polyclonal rabbit serum in order to detect the neu protein product in tissue sections of tumors. This serum specifically reacted with a 185 kilodaltons neu protein in SKBR-3 cells, a mammary carcinoma cell line with amplified neu. Immunohistochemistry on paraffin-embedded sections of tumors in which the neu gene was amplified showed distinct membrane staining of groups of tumor cells. Sections of tumors with normal copy numbers of neu were negative. Lymph node metastases from tumors positive for neu overexpression also showed the membrane staining pattern, whereas lymph node metastases from tumors negative for neu staining never did. Neu amplification is thus associated with neu protein overproduction in tumors and lymph node metastases, and a routine antibody staining technique can discriminate a high level of neu protein expression from levels commonly present in tumors with normal neu copy numbers.
neu（或c-erbB-2或HER）癌基因在人类乳腺癌中相对常见。我们制备了一种多克隆兔血清，以检测肿瘤组织切片中的neu蛋白产物。该血清与SKBR-3细胞（一种neu基因扩增的乳腺癌细胞系）中的185千道尔顿neu蛋白发生特异性反应。对neu基因扩增的肿瘤石蜡包埋切片进行免疫组织化学检测，结果显示肿瘤细胞群有明显的膜染色。neu拷贝数正常的肿瘤切片呈阴性。neu过表达阳性的肿瘤的淋巴结转移灶也呈现膜染色模式，而neu染色阴性的肿瘤的淋巴结转移灶则从未出现这种情况。因此，neu扩增与肿瘤及淋巴结转移灶中neu蛋白的过量产生有关，并且常规抗体染色技术能够区分高水平的neu蛋白表达与neu拷贝数正常的肿瘤中常见的表达水平。
