A、B及D群链球菌的初步鉴定
Presumptive identification of group A, B, and D streptococci.
作者信息
Facklam R R, Padula J F, Thacker L G, Wortham E C, Sconyers B J
出版信息
Appl Microbiol. 1974 Jan;27(1):107-13. doi: 10.1128/am.27.1.107-113.1974.
Abstract
A battery of five tests was used for presumptive identification of the pathogenic streptococci. The non-serological methods included determination of hemolysis for all strains, bacitracin susceptibility for group A streptococci, hippurate hydrolysis by group B streptococci, and bile-esculin reaction for group D streptococci. Enterococcal group D streptococci were differentiated from non-enterococcal group D streptococci by 6.5% NaCl tolerance. Two other categories of streptococci resulted: beta-hemolytic streptococci non-groups A, B, or D; and alpha- or nonhemolytic streptococci, not enterococci, not further identified (viridans streptococci). The tests were used as a battery and not as single entities. In this manner more than 99% of the group A, 99% of the group B, 81% of the beta-hemolytic streptococci non-group A, B, or D, 99% of the group D enterococci, 97% of the group D non-enterococci, and 94% of the viridans streptococci were correctly identified.
摘要
使用一组五项试验对致病性链球菌进行初步鉴定。非血清学方法包括测定所有菌株的溶血情况、A 组链球菌对杆菌肽的敏感性、B 组链球菌的马尿酸盐水解以及 D 组链球菌的胆汁-七叶苷反应。通过 6.5%氯化钠耐受性将肠球菌 D 组链球菌与非肠球菌 D 组链球菌区分开来。还产生了另外两类链球菌：A、B 或 D 组以外的β溶血性链球菌；以及α或非溶血性链球菌（非肠球菌，未进一步鉴定，即草绿色链球菌）。这些试验是作为一组使用的而非单个使用。通过这种方式，超过 99%的 A 组、99%的 B 组、81%的 A、B 或 D 组以外的β溶血性链球菌、99%的 D 组肠球菌、97%的 D 组非肠球菌以及 94%的草绿色链球菌被正确鉴定。
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