Facklam R R, Thacker L G, Fox B, Eriquez L
J Clin Microbiol. 1982 Jun;15(6):987-90. doi: 10.1128/jcm.15.6.987-990.1982.
A test is described that could replace bacitracin susceptibility for presumptive identification of group A streptococci as well as 6.5% NaCl agar tolerance for presumptive identification of enterococcal streptococci. The L-pyrrolidonyl-beta-naphthylamide test, based on hydrolysis of pyrrolidonyl-beta-naphthylamide, was used in conjunction with the CAMP and bile-esculin tests to presumptively identify the streptococci. Among the beta-hemolytic streptococci; 98% of 50 group A, 98% of 46 group B, and 100% of 70 strains that were not group A, B, or D were correctly identified by the new presumptive test scheme. Among the non-beta-hemolytic streptococci; 96% of 74 group D enterococcal, 100% of 30 group D nonenterococcal, and 82% of 112 viridans strains were correctly identified by the new presumptive test scheme.
本文描述了一种检测方法，它可替代杆菌肽敏感性试验用于A群链球菌的初步鉴定，以及替代6.5%氯化钠琼脂耐受试验用于肠球菌属链球菌的初步鉴定。基于吡咯烷酮-β-萘酰胺水解的L-吡咯烷酮-β-萘酰胺试验与CAMP试验和胆汁七叶苷试验联合使用，用于初步鉴定链球菌。在β溶血性链球菌中，新的初步检测方案能正确鉴定50株A群链球菌中的98%、46株B群链球菌中的98%以及70株非A、B或D群菌株中的100%。在非β溶血性链球菌中，新的初步检测方案能正确鉴定74株D群肠球菌中的96%、30株D群非肠球菌中的100%以及112株草绿色链球菌中的82%。
