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用于鉴定金黄色葡萄球菌的凝固酶、脱氧核糖核酸酶(DNase)和热稳定核酸酶试验的比较。

Comparison of coagulase, deoxyribonuclease (DNase), and heat-stable nuclease tests for identification of Staphylococcus aureus.

作者信息

Menzies R E

出版信息

J Clin Pathol. 1977 Jul;30(7):606-8. doi: 10.1136/jcp.30.7.606.

DOI:10.1136/jcp.30.7.606
PMID:328535
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC476495/
Abstract

One thousand and thirty-five clinical isolates of the genus staphylococcus were used to compare the coagulase test with the deoxyribonuclease (DNase) and heat-stable nuclease tests as methods for identifying Staphylococcus aureus. Conflicting results were obtained with 65 isolates when the coagulase test was compared with the DNase test but with only one isolate when the coagulase test was compared with the heat-stable nuclease test. The heat-stable nuclease test produced reliable results after four hours' incubation and was considered a satisfactory substitute for the coagulase test in the clinical laboratory.

摘要

使用1035株葡萄球菌属临床分离株,将凝固酶试验与脱氧核糖核酸酶(DNase)试验及热稳定核酸酶试验进行比较,作为鉴定金黄色葡萄球菌的方法。当凝固酶试验与DNase试验比较时,65株分离株出现了相互矛盾的结果,但当凝固酶试验与热稳定核酸酶试验比较时,只有1株分离株出现矛盾结果。热稳定核酸酶试验在孵育4小时后产生了可靠的结果,被认为是临床实验室中凝固酶试验的令人满意的替代方法。

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本文引用的文献

1
THE CLASSIFICATION OF STAPHYLOCOCCI AND MICROCOCCI FROM WORLD-WIDE SOURCES.来自世界各地的葡萄球菌和微球菌的分类
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Two coagulase-variant forms of Staphylococcus aureus isolated from blood cultures.从血培养中分离出的两种凝固酶变异型金黄色葡萄球菌。
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Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing.通过热核酸酶检测快速鉴定血培养中的金黄色葡萄球菌。
J Clin Microbiol. 1983 Sep;18(3):722-4. doi: 10.1128/jcm.18.3.722-724.1983.
8
Evaluation of various rapid agglutination methods for the identification of Staphylococcus aureus.用于鉴定金黄色葡萄球菌的各种快速凝集方法的评估。
J Clin Microbiol. 1985 May;21(5):726-9. doi: 10.1128/jcm.21.5.726-729.1985.
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Rapid assay for in situ identification of coagulase-positive staphylococci recovered by membrane filtration from swimming pool water.通过膜过滤从游泳池水中回收凝固酶阳性葡萄球菌的原位快速鉴定检测法。
Appl Environ Microbiol. 1986 Sep;52(3):589-90. doi: 10.1128/aem.52.3.589-590.1986.
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Comparative evaluation of identification systems for testing methicillin-resistant strains of Staphylococcus aureus.用于检测耐甲氧西林金黄色葡萄球菌菌株的鉴定系统的比较评估。
J Clin Microbiol. 1986 Aug;24(2):173-6. doi: 10.1128/jcm.24.2.173-176.1986.
Appl Microbiol. 1971 May;21(5):823-6. doi: 10.1128/am.21.5.823-826.1971.
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Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.用于检测葡萄球菌核酸酶活性的异染琼脂扩散法。
Appl Microbiol. 1971 Apr;21(4):585-7. doi: 10.1128/am.21.4.585-587.1971.
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Deoxyribonuclease-positive Staphylococcus epidermidis strains.脱氧核糖核酸酶阳性表皮葡萄球菌菌株
Appl Microbiol. 1970 Jul;20(1):54-7. doi: 10.1128/am.20.1.54-57.1970.
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Reassessment of the coagulase and thermostable nuclease tests as means of identifying Staphylococcus aureus.重新评估凝固酶和耐热核酸酶试验作为鉴定金黄色葡萄球菌的方法。
Appl Microbiol. 1975 Apr;29(4):451-4. doi: 10.1128/am.29.4.451-454.1975.