重新评估凝固酶和耐热核酸酶试验作为鉴定金黄色葡萄球菌的方法。
Reassessment of the coagulase and thermostable nuclease tests as means of identifying Staphylococcus aureus.
作者信息
Rayman M K, Park C E, Philpott J, Todd E C
出版信息
Appl Microbiol. 1975 Apr;29(4):451-4. doi: 10.1128/am.29.4.451-454.1975.
Abstract
A total of 91 enterotoxigenic strains of Staphylococcus auerus isolated from foods and tested for production of coagulase and thermostable nuclease and the ability to ferment glucose and mannitol showed, with the exception of four strains, a complete correlation among these properties. A similar correlation was observed with 103 cultures of S. aureus isolated from clinical material. In all instances, the coagulase reactions were sufficiently strong to be scored at either the 3+ or 4+ levels. Presumptive staphylococcal cultures isolated during routine examination of foods and yielding 2+ coagulase reactions or lower were invariably negative for thermostable nuclease production. It is suggested that the thermostable nuclease test be performed on cultures with doubtful coagulase reactions before classifying them as S. aureus.
摘要
从食品中分离出91株产肠毒素的金黄色葡萄球菌，对其进行凝固酶、耐热核酸酶产生能力以及发酵葡萄糖和甘露醇能力的检测，结果显示，除4株菌外，这些特性之间完全相关。从临床材料中分离出的103株金黄色葡萄球菌培养物也观察到类似的相关性。在所有情况下，凝固酶反应都足够强，可评为3+或4+水平。在食品常规检查中分离出的凝固酶反应为2+或更低的推测性葡萄球菌培养物，其耐热核酸酶产生结果均为阴性。建议在将凝固酶反应可疑的培养物归类为金黄色葡萄球菌之前，先对其进行耐热核酸酶检测。
相似文献
1
Reassessment of the coagulase and thermostable nuclease tests as means of identifying Staphylococcus aureus.重新评估凝固酶和耐热核酸酶试验作为鉴定金黄色葡萄球菌的方法。
Appl Microbiol. 1975 Apr;29(4):451-4. doi: 10.1128/am.29.4.451-454.1975.
2
Interpretation of the tube coagulase test for identification of Staphylococcus aureus.用于鉴定金黄色葡萄球菌的试管凝固酶试验解读
Appl Microbiol. 1975 Apr;29(4):502-5. doi: 10.1128/am.29.4.502-505.1975.
3
Comparison of rabbit and pig plasma in the tube coagulase test.兔血浆和猪血浆在试管凝固酶试验中的比较。
J Clin Microbiol. 1977 Feb;5(2):221-4. doi: 10.1128/jcm.5.2.221-224.1977.
4
Deoxyribonucleic acid base composition and biochemical properties of certain coagulase-negative enterotoxigenic cocci.某些凝固酶阴性产肠毒素球菌的脱氧核糖核酸碱基组成及生化特性
Appl Microbiol. 1975 Feb;29(2):152-8. doi: 10.1128/am.29.2.152-158.1975.
5
Evaluation of three test procedures for identification of Staphylococcus aureus from clinical sources.评估三种从临床样本中鉴定金黄色葡萄球菌的检测方法。
J Clin Microbiol. 1978 Feb;7(2):133-6. doi: 10.1128/jcm.7.2.133-136.1978.
6
Rapid characterization of bacteria, with emphasis on Staphylococcus aureus.细菌的快速鉴定，重点是金黄色葡萄球菌。
Can J Microbiol. 1972 Nov;18(11):1623-7. doi: 10.1139/m72-251.
7
[Production of coagulase and thermonuclease in 366 strains of staphylococci belonging to different lyogroups].[366株不同冻干组葡萄球菌中凝固酶和耐热核酸酶的产生]
Boll Ist Sieroter Milan. 1985;64(2):115-8.
8
9
10
Enterotoxin production by staphylococci isolated from foods in France.法国食品中分离出的葡萄球菌产生肠毒素的情况。
Int J Food Microbiol. 1997 Apr 15;35(3):213-21. doi: 10.1016/s0168-1605(96)01234-2.
引用本文的文献
1
Differentiation of coagulase-positive and coagulase-negative staphylococci by lectins and plant agglutinins.利用凝集素和植物凝集素鉴别凝固酶阳性和凝固酶阴性葡萄球菌
J Clin Microbiol. 1982 Apr;15(4):547-53. doi: 10.1128/jcm.15.4.547-553.1982.
2
Evaluation of the latex slide agglutination test for identification of Staphylococcus aureus.用于鉴定金黄色葡萄球菌的乳胶玻片凝集试验的评估
J Clin Microbiol. 1982 Feb;15(2):275-7. doi: 10.1128/jcm.15.2.275-277.1982.
3
Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test.通过乳胶凝集试验快速可靠地鉴定金黄色葡萄球菌。
J Clin Microbiol. 1980 Nov;12(5):641-3. doi: 10.1128/jcm.12.5.641-643.1980.
4
Accelerated procedure for the enumeration and identification of food-borne Staphylococcus aureus.食源金黄色葡萄球菌计数与鉴定的加速程序
Appl Environ Microbiol. 1980 Jan;39(1):17-9. doi: 10.1128/aem.39.1.17-19.1980.
5
Evaluation of latex agglutination tests for establishing coagulase status of staphylococci from milk.乳胶凝集试验用于确定牛奶中葡萄球菌凝固酶状态的评估。
Can J Comp Med. 1984 Apr;48(2):223-4.
6
Comparison of Vogel-Johnson and Baird-Parker media for membrane filtration recovery of staphylococci in swimming pool water.用于膜过滤法回收游泳池水中葡萄球菌的Vogel-Johnson培养基和Baird-Parker培养基的比较。
Appl Environ Microbiol. 1983 Dec;46(6):1318-22. doi: 10.1128/aem.46.6.1318-1322.1983.
7
Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing.通过热核酸酶检测快速鉴定血培养中的金黄色葡萄球菌。
J Clin Microbiol. 1983 Sep;18(3):722-4. doi: 10.1128/jcm.18.3.722-724.1983.
8
Evaluation of various rapid agglutination methods for the identification of Staphylococcus aureus.用于鉴定金黄色葡萄球菌的各种快速凝集方法的评估。
J Clin Microbiol. 1985 May;21(5):726-9. doi: 10.1128/jcm.21.5.726-729.1985.
9
Rapid assay for in situ identification of coagulase-positive staphylococci recovered by membrane filtration from swimming pool water.通过膜过滤从游泳池水中回收凝固酶阳性葡萄球菌的原位快速鉴定检测法。
Appl Environ Microbiol. 1986 Sep;52(3):589-90. doi: 10.1128/aem.52.3.589-590.1986.
10
Medium dependence for rapid detection of thermonuclease activity in blood culture broths.血培养肉汤中热核酸酶活性快速检测的培养基依赖性
J Clin Microbiol. 1986 Sep;24(3):482-3. doi: 10.1128/jcm.24.3.482-483.1986.
本文引用的文献
1
PLASMA COAGULATION BY ORGANISMS OTHER THAN STAPHYLOCOCCUS AUREUS.除金黄色葡萄球菌外的其他生物体引起的血浆凝固
J Bacteriol. 1965 Jan;89(1):101-5. doi: 10.1128/jb.89.1.101-105.1965.
2
Attempt in classification of catalase-positive staphylococci and micrococci.过氧化氢酶阳性葡萄球菌和微球菌的分类尝试。
J Bacteriol. 1962 Dec;84(6):1140-7. doi: 10.1128/jb.84.6.1140-1147.1962.
3
The use of a lyophilized human plasma standardized for blood coagulation factors in the coagulase and fibrinolytic tests.在凝固酶和纤维蛋白溶解试验中使用针对凝血因子标准化的冻干人血浆。
J Lab Clin Med. 1958 Dec;52(6):888-94.
4
Enzymatic detection of the growth of Staphylococcus aureus in foods.食品中金黄色葡萄球菌生长的酶促检测。
Appl Microbiol. 1967 Sep;15(5):1150-9. doi: 10.1128/am.15.5.1150-1159.1967.
5
The micro-slide gel double diffusion test for the detection and assay of staphylococcal enterotoxins.用于检测和测定葡萄球菌肠毒素的微量玻片凝胶双向扩散试验。
Health Lab Sci. 1969 Oct;6(4):185-98.
6
Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.用于检测葡萄球菌核酸酶活性的异染琼脂扩散法。
Appl Microbiol. 1971 Apr;21(4):585-7. doi: 10.1128/am.21.4.585-587.1971.
7
Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus.金黄色葡萄球菌凝固酶、肠毒素及热稳定脱氧核糖核酸酶产生之间的关系。
Appl Microbiol. 1969 Jul;18(1):126-7. doi: 10.1128/am.18.1.126-127.1969.
8
Pour-plate method for the detection of coagulase production by Staphylococcus aureus.用于检测金黄色葡萄球菌凝固酶产生的倾注平板法。
Appl Microbiol. 1973 Apr;25(4):558-61. doi: 10.1128/am.25.4.558-561.1973.
9
Report on microbiological methods.微生物学方法报告。
J Assoc Off Anal Chem. 1974 Mar;57(2):312-4.
10
Detection of nuclease activity in semisolid and broth cultures.半固体和肉汤培养物中核酸酶活性的检测。
Appl Microbiol. 1969 Aug;18(2):174-6. doi: 10.1128/am.18.2.174-176.1969.
Zotero 插件