评估三种从临床样本中鉴定金黄色葡萄球菌的检测方法。
Evaluation of three test procedures for identification of Staphylococcus aureus from clinical sources.
作者信息
Zarzour J Y, Belle E A
出版信息
J Clin Microbiol. 1978 Feb;7(2):133-6. doi: 10.1128/jcm.7.2.133-136.1978.
Abstract
A total of 520 clinical and environmental isolates of the family Micrococcaceae that fermented glucose anaerobically were tested for their ability to produce coagulase, thermostable nuclease, and deoxyribonuclease. Of these, 450 isolates coagulated rabbit plasma, produced thermostable nuclease, and were identified as Staphylococcus aureus, 447 of which produced a 3+ to 4+ clot. The remaining three isolates produced a 2+ clot, deoxyribonuclease, and thermostable nuclease. It was found that three of the S. aureus isolates failed to produce deoxyribonuclease. A total of 70 isolates which did not coagulate rabbit plasma and which were thermostable nuclease negative were identified as S. epidermidis. Three of them produced deoxyribonuclease. It is suggested that the thermostable nuclease test be performed on all isolates producing a 2+ (or 1+) clot in the coagulase test before identifying them as S. aureus.
摘要
总共对520株在厌氧条件下发酵葡萄糖的微球菌科临床和环境分离株进行了检测，以评估它们产生凝固酶、耐热核酸酶和脱氧核糖核酸酶的能力。其中，450株分离株使兔血浆凝固、产生耐热核酸酶，并被鉴定为金黄色葡萄球菌，其中447株产生3+至4+的凝块。其余三株分离株产生2+的凝块、脱氧核糖核酸酶和耐热核酸酶。发现有三株金黄色葡萄球菌分离株未产生脱氧核糖核酸酶。总共70株未使兔血浆凝固且耐热核酸酶阴性的分离株被鉴定为表皮葡萄球菌。其中三株产生脱氧核糖核酸酶。建议在将所有在凝固酶试验中产生2+（或1+）凝块的分离株鉴定为金黄色葡萄球菌之前，对其进行耐热核酸酶试验。
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