Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB), Laboratório de Biologia Celular e Tecidual (LBCT), Av. Alberto Lamego 2000, 28013-602, Campos Dos Goytacazes, RJ, Brazil.
UENF, CBB, Laboratório de Química e Função de Proteínas e Peptídeos, Campos dos Goytacazes, RJ, Brazil.
Biochim Biophys Acta Proteins Proteom. 2020 Dec;1868(12):140529. doi: 10.1016/j.bbapap.2020.140529. Epub 2020 Aug 25.
The light spectrum quality is an important signal for plant growth and development. We evaluated the effects of different light spectra on the in vitro shoot development of Cedrela fissilis and its proteomic and polyamine (PA) profiles. Cotyledonary and apical nodal segments were grown under different light emitting diodes (LED) and fluorescent lamps. Shoots from cotyledonary nodal segments cultured with 6-benzyladenine (BA) that were grown under WBR LED showed increased length and higher fresh and dry matter compared to shoots grown under fluorescent lamps. A nonredundant protein databank generated by transcriptome sequencing and the de novo assembly of C. fissilis improved, and almost doubled, the protein identification compared to a Citrus sinensis databank. A total of 616 proteins were identified, with 23 up- and 103 down-accumulated in the shoots under WBR LEDs compared to fluorescent lamps. Most differentially accumulated proteins in shoots grown under the WmBdR LED lamp treatment compared to the fluorescent lamp treatment are involved in responding to metabolic processes, stress, biosynthetic and cellular protein modifications, and light stimulus processes. Among the proteins, the up-accumulation of argininosuccinate synthase was associated with an increase in the free putrescine content and, consequently, with higher shoot elongation under WBR LED. The down-accumulation of calreticulin, heat shock proteins, plastid-lipid-associated protein, ubiquitin-conjugating enzymes, and ultraviolet-B receptor UVR8 isoform X1 could be related to the longer shoot length noted under LED treatment. This study provides important data related to the effects of the light spectrum quality on in vitro morphogenesis through the modulation of specific proteins and free putrescine biosynthesis in C. fissilis, an endangered wood species from the Brazilian Atlantic Forest of economic and ecological relevance. The nonredundant protein databank of C. fissilis is available via ProteomeXchange under identifier PXD018020.
光谱质量是植物生长发育的一个重要信号。我们评估了不同光谱对破布木离体芽发育的影响及其蛋白质组学和多胺（PA）谱。子叶和顶端节段在不同的发光二极管（LED）和荧光灯下生长。在 WBR LED 下培养的含有 6-苄基腺嘌呤（BA）的子叶节段的芽与在荧光灯下生长的芽相比，长度增加，鲜重和干重更高。通过转录组测序和破布木从头组装生成的非冗余蛋白质数据库，与柑橘数据库相比，蛋白质鉴定几乎增加了一倍。共鉴定出 616 种蛋白质，其中在 WBR LED 下生长的芽中有 23 种上调和 103 种下调。与荧光灯处理相比，在 WmBdR LED 灯下生长的芽中，大多数差异积累的蛋白质参与代谢过程、应激、生物合成和细胞蛋白质修饰以及光刺激过程的响应。在这些蛋白质中，精氨酸琥珀酸合酶的上调与游离腐胺含量的增加有关，因此，在 WBR LED 下芽伸长率更高。钙网蛋白、热休克蛋白、质体脂相关蛋白、泛素连接酶和紫外线-B 受体 UVR8 同工型 X1 的下调积累可能与 LED 处理下观察到的更长的芽长度有关。本研究通过调节特定蛋白质和游离腐胺生物合成，为光谱质量对破布木离体形态发生的影响提供了重要数据，破布木是一种具有经济和生态意义的巴西大西洋森林濒危木材物种。破布木的非冗余蛋白质数据库可通过 ProteomeXchange 以标识符 PXD018020 获得。
