Abdouli Dhekra, Soufi Sihem, Bettaieb Taoufik, Werbrouck Stefaan P O
Laboratory for Applied In Vitro Plant Biotechnology, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium.
Laboratory of Horticultural Sciences, National Agronomic Institute of Tunisia, University of Carthage, 43 Av. Charles Nicolle, Tunis 1082, Tunisia.
Plants (Basel). 2023 Apr 3;12(7):1546. doi: 10.3390/plants12071546.
Light-emitting diodes (LEDs) are popular as a light source for in vitro plants because they save energy and allow the morphology of the plant to be altered. The purpose of this study was to show that switching from classical fluorescent light (FL) to LED light can have both beneficial and adverse effects. plantlets were exposed to FL, monochromatic Blue LED light (B), monochromatic Red LED light (R), and a 1:1 mixture of both B and R (BR). R increased the total weight, shoot length, number of shoots ≥ 1 cm, and proliferation. It also reduced hyperhydricity (HH), but also dramatically increased shoot tip necrosis (STN) and leaf necrosis (LN). B cured plants of HH and STN, but hardly enabled proliferation. It did not solve the problem of LN, but the plants were high in total chlorophyll and carotenoids. BR reduced HH but enabled limited proliferation, high STN, and LN. All three LED treatments reduced HH compared to FL. B induced both high total phenolic and flavonoid content and high DPPH-scavenging activity. These results show that switching from FL to LED can have a significant positive or negative effect on proliferation and quality. This suggests that finding an optimal lighting regimen will take a lot of trial and error.
发光二极管（LED）作为体外培养植物的光源很受欢迎，因为它们节能且能改变植物的形态。本研究的目的是表明从传统荧光灯（FL）切换到LED灯可能既有有益影响也有不利影响。将幼苗暴露于FL、单色蓝色LED灯（B）、单色红色LED灯（R）以及B和R的1:1混合物（BR）下。R增加了总重量、茎长、≥1厘米的茎数和增殖。它还降低了玻璃化（HH），但也显著增加了茎尖坏死（STN）和叶片坏死（LN）。B治愈了植物的HH和STN，但几乎不能促进增殖。它没有解决LN问题，但植物的总叶绿素和类胡萝卜素含量很高。BR降低了HH，但增殖有限，STN和LN较高。与FL相比，所有三种LED处理都降低了HH。B诱导了高总酚和类黄酮含量以及高DPPH清除活性。这些结果表明，从FL切换到LED可能对增殖和质量产生显著的正面或负面影响。这表明找到最佳照明方案将需要大量的反复试验。
