Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal De Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
Oral Pathology Section, School of Dentistry, Pontifical Catholic University of Minas Gerais (PUC Minas), Belo Horizonte, MG, Brazil.
Pathol Res Pract. 2020 Oct;216(10):153138. doi: 10.1016/j.prp.2020.153138. Epub 2020 Jul 26.
Tumor budding (TB) is a promising prognostic marker in many cancers including oral squamous cell carcinoma. The evaluation of TB in preoperative diagnostic biopsies has been proven be possible; therefore, the association of TB with other morphological features can represent an important aid in the previous treatment decision. This study aims to evaluate TB in oral squamous cell carcinoma (OSCC) biopsies, assessing its association with other morphological characteristics of the sample. A total of 56 cases of OSCC were investigated. In hematoxylin and eosin-stained slides, morphological features including histopathological grading and mode of invasion were evaluated in the deep invasive front. Moreover, immunohistochemistry was performed with anti-multi-cytokeratin antibody helping in the identification of TB, which was graded as low-intensity or no TB and high-intensity TB. Descriptive and bivariate analyses were performed, and the level of significance was set at 5%. The tongue was the most-affected site with 29 (51.7 %) tumors. The predominant mode of invasion (27-48.2 %) was by groups of neoplastic cells without clear boundaries. Of the cases investigated, 37 (66.1 %) were high-intensity TB, which was associated with the mode of invasion of the tumors (p < 0.05). All cases with the worst mode of invasion showed high-intensity TB. Preliminary results showed the potential of morphological features, such as TB and mode of invasion, evaluated in diagnostic specimens of OSCC, aiding in the treatment decision to select patients who could benefit from more-aggressive treatments.
肿瘤芽（TB）是许多癌症包括口腔鳞状细胞癌的一种很有前途的预后标志物。在术前诊断性活检中评估 TB 已被证明是可行的；因此，TB 与其他形态特征的关联可以代表治疗前决策的重要辅助手段。本研究旨在评估口腔鳞状细胞癌（OSCC）活检中的 TB，评估其与样本其他形态特征的相关性。共研究了 56 例 OSCC 病例。在苏木精和伊红染色的切片中，在深部浸润前沿评估了形态特征，包括组织病理学分级和浸润方式。此外，还进行了抗多细胞角蛋白抗体的免疫组织化学染色，有助于识别 TB，TB 分为低强度或无 TB 和高强度 TB。进行了描述性和双变量分析，显著性水平设置为 5%。受影响最严重的部位是舌头，有 29 个（51.7%）肿瘤。最主要的浸润方式（27-48.2%）是肿瘤细胞群无明确边界的浸润。在所研究的病例中，37 例（66.1%）为高强度 TB，与肿瘤浸润方式有关（p<0.05）。所有浸润方式最差的病例均显示高强度 TB。初步结果显示，在 OSCC 诊断标本中评估 TB 和浸润方式等形态特征，有助于治疗决策，选择可能受益于更积极治疗的患者。
