Department of Ophthalmology, University Hospital Würzburg, Würzburg, Germany.
Department of Computer Science, University of Alabama at Birmingham, Birmingham, AL, USA.
Transl Vis Sci Technol. 2020 Jul 1;9(8):2. doi: 10.1167/tvst.9.8.2. eCollection 2020 Jul.
To use multimodal retinal images (including quantitative fundus autofluorescence [QAF]) for spectral-domain optical coherence tomography (SD-OCT)-based image registration and alignment. For each age decade of healthy adults, normative fine-grained QAF retinal maps are generated and advanced methods for QAF image analysis are applied.
Multimodal retinal images were obtained from 103 healthy subjects (age 19-77 years; unremarkable retina/macula, age-appropriate clear optic media). Custom written FIJI plugins enabled: (1) determination of the fovea in SD-OCT and the edge of the optic disc in infrared (IR) images; (2) alignment and superimposition of multimodal retinal images based on foveal and optic disc position; (3) plotting of normative QAF retinal maps for each decade; and (4) comparison of individual retinas with normative retinas of different decades using newly introduced analysis patterns (QAF97, freehand tool).
SD-OCT based image registration enables easy image registration, alignment, and analysis of different modalities (QAF, IR, and SD-OCT here reported). In QAF, intensities significantly increase with age with two major inclines between the third/fourth and seventh/eighth decades. With aging, the parafoveal area of maximum QAF intensity slightly shifts from temporal-superior to temporal. Compared with standard QAF analysis, refined QAF analysis patterns reveal a more detailed analysis of QAF, especially in the diseased retina.
Age-related QAF normative retinal maps can be used to directly compare and classify individual's QAF intensities. Advanced QAF analysis tools will further help to interpret autofluorescence changes in normal aging and in the diseased retina in a multimodal imaging setting.
Advanced methods for QAF analysis link basic findings with clinical observations in normal aging and in the diseased macula.
使用多模态视网膜图像（包括定量眼底自发荧光 [QAF]）进行基于谱域光相干断层扫描（SD-OCT）的图像配准和对齐。为每个年龄段的健康成年人生成规范的精细 QAF 视网膜图，并应用先进的 QAF 图像分析方法。
从 103 名健康受试者（年龄 19-77 岁；无明显视网膜/黄斑，年龄适当的清晰眼内介质）中获得多模态视网膜图像。编写的 FIJI 插件实现了以下功能：（1）在 SD-OCT 中确定黄斑中心凹，在红外（IR）图像中确定视盘边缘；（2）基于黄斑中心凹和视盘位置对齐和叠加多模态视网膜图像；（3）为每个十年绘制规范的 QAF 视网膜图；（4）使用新引入的分析模式（QAF97，徒手工具）将个体视网膜与不同十年的正常视网膜进行比较。
基于 SD-OCT 的图像配准可轻松实现不同模态（此处报告的 QAF、IR 和 SD-OCT）的图像配准、对齐和分析。在 QAF 中，强度随年龄显著增加，在第三/四十年和第七/八十年之间有两个主要的上升。随着年龄的增长，最大 QAF 强度的旁中心区域从颞上向颞下略微移位。与标准 QAF 分析相比，精细的 QAF 分析模式可更详细地分析 QAF，尤其是在病变视网膜中。
与年龄相关的 QAF 规范视网膜图可用于直接比较和分类个体的 QAF 强度。先进的 QAF 分析工具将有助于在多模态成像环境中解释正常衰老和病变视网膜中的自发荧光变化。
邹宇豪
