iTRAQ 基于的比较蛋白质组学分析揭示了各种肾脏疾病的特异性尿生物标志物。

iTRAQ-based comparative proteomics analysis reveals specific urinary biomarkers for various kidney diseases.

机构信息

Department of Nephrology, Zhejiang Provincial People's Hospital, Zhejiang 310014, PR China.

Department of Nephrology, People's Hospital of Hangzhou Medical College, Zhejiang 310014, PR China.

出版信息

Biomark Med. 2020 Jul;14(10):839-854. doi: 10.2217/bmm-2019-0556. Epub 2020 Aug 28.

DOI:10.2217/bmm-2019-0556

PMID:32856461

Abstract

Proteome studies for multiple renal diseases is bare. Using isobaric tags for relative and absolute quantitation labeling, many differentially expressed proteins (DEPs) were identified in acute kidney injury (AKI), AKI + chronic kidney disease (CKD), diabetic CKD and nondiabetic CKD with or without IgA nephropathy (IgAN). Comparative analysis indicated that 34, 35, 17, 91 and 14 unique DEPs were found in AKI, AKI + CKD, CKD, diabetic CKD and nondiabetic CKD. Compared with nondiabetic CKD with IgAN, 47 unique DEPs were found in that without IgAN. Serum amyloid A1 (SAA1) and hepatocyte growth factor activator were unregulated in AKI and nondiabetic CKD without IgAN, respectively. Regenerating islet-derived protein 3-α (Reg3A) upregulation is associated with AKI and AKI + CKD patients. This research contributes to urinary biomarker discovery from multiple renal diseases.

摘要

多种肾脏疾病的蛋白质组学研究还很少。使用相对和绝对定量标记的等压标签，在急性肾损伤 (AKI)、AKI+慢性肾脏病 (CKD)、糖尿病 CKD 和非糖尿病 CKD 中发现了许多差异表达蛋白 (DEPs)，这些 CKD 患者伴有或不伴有 IgA 肾病 (IgAN)。比较分析表明，AKI、AKI+CKD、CKD、糖尿病 CKD 和非糖尿病 CKD 中分别发现了 34、35、17、91 和 14 个独特的 DEPs。与伴有 IgAN 的非糖尿病 CKD 相比，在不伴有 IgAN 的非糖尿病 CKD 中发现了 47 个独特的 DEPs。血清淀粉样蛋白 A1 (SAA1) 和肝细胞生长因子激活物在 AKI 和非糖尿病 CKD 中无 IgAN 时分别上调。再生胰岛衍生蛋白 3-α (Reg3A) 的上调与 AKI 和 AKI+CKD 患者相关。这项研究有助于从多种肾脏疾病中发现尿液生物标志物。