Team of Ethnopharmacology and Pharmacognosy, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University of Meknes, BP 509, Boutalamine, Errachidia, 52000, Morocco.
Cardiovasc Hematol Disord Drug Targets. 2020;20(4):305-316. doi: 10.2174/1871529X20666200827113029.
The aim of this study was to evaluate the antidiabetic effect of Scorzonera undulata.
Scorzonera undulata ssp deliciosa, locally known as "Guiz", is used as a phytomedicine in Morocco and Algeria to treat different health problems. Interestingly, it is used in the Moroccan pharmacopeia to treat diabetes. To our knowledge, this medicinal herb has never been investigated for any pharmacological activity.
This study aimed to evaluate the antihyperglycemic effect of the aqueous extract of the aerial part of Scorzonera undulata (SUAP) in normal and STZ-induced diabetic rats and to assess the acute toxicity of this extract in Wistar rats.
This study investigated the effects of SUAP at a dose of 20 mg/kg on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rats. The acute toxicity of SUAP was examined according to the OECD test guideline; rats were divided into four groups of each sex and orally received the SUAP (1000, 2000, or 3000 mg/kg BW). Post-treatment, body weight, signs of toxicity, and/or mortality were observed during 14 days. Other assays such as histopathological examination, preliminary phytochemical investigation, determination of glycogen content and evaluation of α-amylase were performed according to standard protocols.
The findings of the current study depicted that both single and repeated oral administration of SUAP (20 mg/kg) generated a significant fall in the blood glucose levels in diabetic rats. A single oral administration of SUAP (at the highest dose of 3000 mg/kg BW) had no significant acute toxicological effects, and oral LD50 of SUAP was greater than 3000 mg/kg. Furthermore, repeated oral administration of SUAP during 15 days led to an increase in the liver glycogen content in diabetic rats to improve the histopathological structure of the liver and pancreas in SUAPtreated diabetic rats and to ameliorate some biochemical parameters such as ALT and creatinine. SUAP had no effect on α-amylase activity. In addition, the preliminary phytochemical investigation showed the richness of the roots of SUAP in some phytochemicals, particularly the polyphenols.
The present study demonstrates the antihyperglycemic effect of Scorzonera undulata in diabetic rats which could be involved through the improvement of liver structure and function. In addition, the dose used is not toxic. Finally, the extract contains large amounts of bioactive compounds, mainly polyphenols.
本研究旨在评估獐牙菜的降血糖作用。
獐牙菜属植物 ssp 美味，在摩洛哥和阿尔及利亚当地被称为“Guiz”，用作草药治疗各种健康问题。有趣的是，它在摩洛哥药典中被用于治疗糖尿病。据我们所知，这种药草从未被研究过任何药理学活性。
本研究旨在评估獐牙菜地上部分水提物（SUAP）在正常和 STZ 诱导的糖尿病大鼠中的降血糖作用，并评估该提取物在 Wistar 大鼠中的急性毒性。
本研究考察了 SUAP 在 20mg/kg 剂量下对正常和链脲佐菌素（STZ）诱导的糖尿病大鼠血糖水平的影响。根据 OECD 试验指南，考察了 SUAP 的急性毒性；将大鼠分为每组雌雄各一组，口服给予 SUAP（1000、2000 或 3000mg/kgBW）。治疗后，观察 14 天内的体重、毒性体征和/或死亡率。根据标准方案进行组织病理学检查、初步植物化学研究、糖原含量测定和α-淀粉酶评价等其他检测。
本研究发现，单次和重复口服 SUAP（20mg/kg）均可显著降低糖尿病大鼠的血糖水平。单次口服 SUAP（最高剂量 3000mg/kgBW）无明显急性毒理学作用，口服 SUAP 的 LD50 大于 3000mg/kg。此外，重复口服 SUAP 15 天可增加糖尿病大鼠肝糖原含量，改善 SUAP 治疗糖尿病大鼠的肝和胰腺组织的组织病理学结构，改善 ALT 和肌酐等一些生化参数。SUAP 对α-淀粉酶活性无影响。此外，初步植物化学研究表明，SUAP 根富含一些植物化学物质，特别是多酚。
本研究表明，獐牙菜在糖尿病大鼠中具有降血糖作用，这可能是通过改善肝脏结构和功能实现的。此外，所用剂量无毒性。最后，提取物含有大量的生物活性化合物，主要是多酚。
