Peng Xiandong, Yu Min, Li Lu, Fu Wei, Chen Hua, Sun Xiaoxi, Chen Jiazhou
Shanghai JIAI Genetics & IVF Institute, Obstetrics & Gynecology Hospital of Fudan University, Shanghai 200011, China.
Shanghai JIAI Genetics & IVF Institute, Obstetrics & Gynecology Hospital of Fudan University, Shanghai 200011, China; Shanghai Key Laboratory for Female Reproductive Endocrine-Related Diseases, Institute of Reproductive and Development, Fudan University, Shanghai 200433, China.
Reprod Biol. 2020 Dec;20(4):496-500. doi: 10.1016/j.repbio.2020.08.002. Epub 2020 Aug 26.
Blastocyst culture and transplantation have now been widely used in most reproductive centers. Our aim was to explore the effects of euploid blastocyst morphological development on the reproductive outcomes of frozen-thawed euploid blastocysts on the fifth day or sixth day. The retrospective analysis included the clinical data of 849 patients who underwent euploid blastocyst transplantation between January 2014 and January 2018. The patients were divided into well-developed blastocysts (BB) or poorly developed blastocysts (BC, CB) groups according to blastocyst morphology grade. The intracytoplasmic sperm injection (ICSI) was carried out on the day of egg retrieval. Morphological evaluation of the resulting blastocysts was performed on Day5 or Day6 of in vitro culture. The pregnancy rate, cumulative pregnancy rate, live birth rate and cumulative live birth rate were the highest in the BB group (P ≤ 0.001). In addition, not only the pregnancy rate of CB group Day5 was lower than that of the BB (P = 0.005) and BC (P = 0.042) groups, but the live birth rate in CB group was lower than BB (P = 0.001) and BC (P = 0.007) groups. However, there was no difference in miscarriage rate among the three groups (P = 0.154). Furthermore, the miscarriage rate of BB (P = 0.048) and BC (P = 0.019) groups at the Day5 cycle was lower than the Day6 cycle. Blastocyst morphological development has positive effects on pregnancy rate and live birth rate, but has no effect on miscarriage rate.
囊胚培养和移植目前已在大多数生殖中心广泛应用。我们的目的是探讨整倍体囊胚形态发育对第5天或第6天冻融整倍体囊胚生殖结局的影响。回顾性分析纳入了2014年1月至2018年1月期间接受整倍体囊胚移植的849例患者的临床资料。根据囊胚形态学分级，将患者分为发育良好的囊胚（BB）组或发育不良的囊胚（BC、CB）组。取卵当天进行卵胞浆内单精子注射（ICSI）。在体外培养的第5天或第6天对所得囊胚进行形态学评估。BB组的妊娠率、累积妊娠率、活产率和累积活产率最高（P≤0.001）。此外，不仅CB组第5天的妊娠率低于BB组（P = 0.005）和BC组（P = 0.042），而且CB组的活产率低于BB组（P = 0.001）和BC组（P = 0.007）。然而，三组间流产率无差异（P = 0.154）。此外，BB组（P = 0.048）和BC组（P = 0.019）在第5周期的流产率低于第6周期。囊胚形态发育对妊娠率和活产率有积极影响，但对流产率无影响。
