Zhang Qian, Huang Rui, Hu Hanqing, Yu Lei, Tang Qingchao, Tao Yangbao, Liu Zheng, Li Jiaying, Wang Guiyu
Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, China.
Department of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100000, China.
iScience. 2020 Aug 14;23(9):101460. doi: 10.1016/j.isci.2020.101460. eCollection 2020 Sep 25.
Hypoxia is serving crucial roles in cancers. This study aims to comprehensively analyze the molecular features and clinical relevance of a well-defined hypoxia-associated signature in pan-cancer using multi-omics data. Data were acquired from TCGA, CCLE, GDSC, and GEO. RNA expression pattern, copy number variation (CNV), methylation, and mutation of the signature were analyzed. The majority of the 15 genes were upregulated in cancer tissues compared with normal tissue, and RNA expression was negatively associated with methylation level. CNV occurred in almost all the cancers, whereas mutation frequency was low across different cancer types. The signature was also closely related to cancer hallmarks and cancer-related metabolism pathways. NDRG1 was upregulated in kidney cancer tissues as indicated by immunohistochemistry. Besides, most of the 15 genes were risk factors for patients' overall survival. Our results provide a valuable resource that will guide both mechanistic and therapeutic analyses of the hypoxia signature in cancers.
缺氧在癌症中发挥着关键作用。本研究旨在利用多组学数据全面分析泛癌中一个明确的缺氧相关特征的分子特征和临床相关性。数据来自TCGA、CCLE、GDSC和GEO。分析了该特征的RNA表达模式、拷贝数变异（CNV）、甲基化和突变情况。与正常组织相比，15个基因中的大多数在癌组织中上调，且RNA表达与甲基化水平呈负相关。几乎所有癌症中都发生了CNV，而不同癌症类型的突变频率较低。该特征还与癌症特征和癌症相关代谢途径密切相关。免疫组织化学显示，NDRG1在肾癌组织中上调。此外，15个基因中的大多数是患者总生存的危险因素。我们的结果提供了一个有价值的资源，将指导癌症中缺氧特征的机制和治疗分析。
