嗜酸乳杆菌亚种 PTCC1057 对链脲佐菌素诱导的糖尿病小鼠血糖、胎球蛋白-A 和 sestrin3 水平的影响。
Effect of Lactobacillus delbrueckii Subsp. lactis PTCC1057 on Serum Glucose, Fetuin-A ,and Sestrin 3 Levels in Streptozotocin-Induced Diabetic Mice.
机构信息
Department of Clinical Biochemistry, Maragheh University of Medical Sciences, Maragheh, Iran.
Department of Microbiology, Higher Education Institute of Rabe Rashid, East Azerbaijan, Tabriz, Iran.
出版信息
Probiotics Antimicrob Proteins. 2021 Apr;13(2):383-389. doi: 10.1007/s12602-020-09693-0.
Intake of probiotic bacteria may improve or preserve insulin sensitivity. Fetuin-A and sestrin 3 have emerged as promising candidate biomarkers for crucial roles in insulin signaling pathway. Therefore, the effect of oral supplementation with the probiotic bacterium Lactobacillus delbrueckii subsp. lactis PTCC1057 on proteins involved in insulin signaling pathway was investigated in normal and streptozotocin (STZ)-induced diabetic mice. The 6-8-week-old female mice were divided into a non-diabetic control, diabetic control, and diabetic experimental and non-diabetic experimental groups (5 mice each group). Diabetic and non-diabetic experimental groups treated with 3 × 10 CFU mL L. delbrueckii subsp. lactis PTCC1057 by gavage feeding approach daily for 28 days. Serum glucose, fetuin-A, and sestrin 3 levels were measured by standard methods. The result showed that oral administration of L. delbrueckii significantly decreased serum glucose in comparison to diabetic control group (P = 0.01). Serum fetuin-A level was higher in diabetic control group than non-diabetic group and oral administration of L. delbrueckii subsp. lactis PTCC1057 significantly decreased fetuin-A level in diabetic experimental group in comparison with non-diabetic groups (P = 0.001). Sestrin 3 level significantly was lower in diabetic control group than non-diabetic control group (P = 0.03) and it significantly increased in diabetic experimental group in comparison with diabetic control group after intervention of L. delbrueckii subsp. lactis PTCC1057 (P = 0.02). The results show that feeding the STZ-induced diabetic mice with L. delbrueckii subsp. lactis PTCC1057 terminated to decrease in fasting blood glucose and fetuin-A level and increase in serum sestrin 3 level. Therefore, the L. delbrueckii subsp. lactis PTCC1057 can be considered as excellent candidate for future studies on diabetes mellitus.
摄入益生菌可能会改善或维持胰岛素敏感性。胎球蛋白-A 和 sestrin 3 已成为胰岛素信号通路中关键作用的有前途的候选生物标志物。因此，研究了口服补充益生菌 Lactobacillus delbrueckii subsp. lactis PTCC1057 对正常和链脲佐菌素 (STZ) 诱导的糖尿病小鼠胰岛素信号通路相关蛋白的影响。将 6-8 周龄的雌性小鼠分为非糖尿病对照组、糖尿病对照组和糖尿病实验组和非糖尿病实验组（每组 5 只）。糖尿病和非糖尿病实验组通过灌胃方式每天用 3×10 CFU mL L 处理。delbrueckii subsp。Lactis PTCC1057 连续 28 天。用标准方法测量血清葡萄糖、胎球蛋白-A 和 sestrin 3 水平。结果表明，与糖尿病对照组相比，口服 L. delbrueckii 显著降低了血清葡萄糖（P=0.01）。糖尿病对照组血清胎球蛋白-A 水平高于非糖尿病对照组，口服 L. delbrueckii subsp。与非糖尿病组相比，Lactis PTCC1057 显著降低了糖尿病实验组的胎球蛋白-A 水平（P=0.001）。与非糖尿病对照组相比，糖尿病对照组 sestrin 3 水平显著降低（P=0.03），经 L. delbrueckii subsp 干预后，糖尿病实验组 sestrin 3 水平显著升高。乳糖 PTCC1057（P=0.02）。结果表明，用 L. delbrueckii subsp。喂养 STZ 诱导的糖尿病小鼠。Lactis PTCC1057 可终止空腹血糖和胎球蛋白-A 水平降低，血清 sestrin 3 水平升高。因此，L. delbrueckii subsp。Lactis PTCC1057 可被视为糖尿病研究的优秀候选者。
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