Jin Shan, Sugitani Iwao
Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical University.
Department of Endocrine Surgery, Graduate School of Medicine, Nippon Medical School.
J Nippon Med Sch. 2021 Sep 1;88(4):311-318. doi: 10.1272/jnms.JNMS.2021_88-409. Epub 2020 Aug 31.
We developed an animal model for evaluating thyroid-stimulating hormone (TSH) suppression therapy after total thyroidectomy in rats.
Sixty Wistar rats were randomly divided into 6 groups, including a sham-operated group (SO group), a total thyroidectomy group (TD group), and a L-thyroxine (L-T4) treatment I group (TS-I group), II group (TS-II group), III group (TS-III group), and IV group (TS-IV group) (in which rats were treated with 1.4, 1.6, 1.8, and 2.0 μg/100 g body weight, respectively) after total thyroidectomy.
HE staining in the TD group and all L-T4-treated rats showed that the resected tissue was normal thyroid gland. No residual thyroid tissue was found in neck tissue of the cross-section of the thyroid gland. Serum T3 levels in the TS-II group were not significantly different from those in the SO group, whereas serum T4 levels were slightly higher than those in the SO group, and serum TSH levels were slightly lower.
Rats subcutaneously injected with L-T4 1.6 μg/100 g body weight for 15 days after total thyroidectomy were suitable as an animal model for TSH suppression therapy.
我们建立了一种用于评估大鼠全甲状腺切除术后促甲状腺激素（TSH）抑制治疗的动物模型。
60只Wistar大鼠随机分为6组，包括假手术组（SO组）、全甲状腺切除组（TD组）以及全甲状腺切除术后L-甲状腺素（L-T4）治疗I组（TS-I组）、II组（TS-II组）、III组（TS-III组）和IV组（TS-IV组）（各组大鼠分别接受1.4、1.6、1.8和2.0μg/100g体重的L-T4治疗）。
TD组及所有L-T4治疗大鼠的HE染色显示切除组织为正常甲状腺组织。甲状腺横断面颈部组织中未发现残留甲状腺组织。TS-II组血清T3水平与SO组无显著差异，而血清T4水平略高于SO组，血清TSH水平略低。
全甲状腺切除术后皮下注射1.6μg/100g体重L-T4共15天的大鼠适合作为TSH抑制治疗的动物模型。
