Li Jun-Bin, Liu Hong-Wen, Fu Ting, Wang Ruowen, Zhang Xiao-Bing, Tan Weihong
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Institute of Molecular Medicine, Renji Hospital, School of Medicine and College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Trends Chem. 2019 May;1(2):224-234. doi: 10.1016/j.trechm.2019.03.002. Epub 2019 Apr 5.
Small-molecule near-IR (NIR) optical imaging has experienced tremendous advancements over the past two decades, playing important roles in both theory and application in the biomedical field. NIR optical imaging affords improved contrast and depth of tissue penetration by reducing photon scattering, lowering tissue absorption, and minimizing autofluorescence in the NIR window. Moreover, molecular engineering endows small-molecule probes with powerful tunability, providing a valuable means for real-time noninvasive visualization of various biological processes and analytes with high sensitivity and resolution. In this review, we focus on the most recent advances in the development of small-molecule NIR probes and their applications in bioimaging. We also highlight the challenges and opportunities in this rapidly developing field.
在过去二十年中，小分子近红外（NIR）光学成像取得了巨大进展，在生物医学领域的理论和应用方面都发挥着重要作用。近红外光学成像通过减少光子散射、降低组织吸收以及最小化近红外窗口中的自发荧光，提高了对比度和组织穿透深度。此外，分子工程赋予小分子探针强大的可调性，为实时无创可视化各种生物过程和分析物提供了一种具有高灵敏度和分辨率的宝贵手段。在本综述中，我们重点关注小分子近红外探针开发的最新进展及其在生物成像中的应用。我们还强调了这个快速发展领域中的挑战和机遇。
