Yang Sha, Tan Xiaofeng, Tang Li, Yang Qinglai
The First Affiliated Hospital and Center for Molecular Imaging Probe, Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, China.
Department of Pathology and Tumor Pathology Research Group, Xiangnan University, Chenzhou, China.
Front Chem. 2021 Nov 15;9:763495. doi: 10.3389/fchem.2021.763495. eCollection 2021.
Near-Infrared-II (NIR-II) bioimaging is a newly emerging visualization modality in real-time investigations of biological processes research. Owning to advances in reducing photon scattering and low tissue autofluorescence levels in NIR-II region (1,000-1700 nm), NIR-II bioimaging affords high resolution with increasing tissue penetration depth, and it shows greater application potential for detection to obtain more detailed qualitative and quantitative parameters. Herein, this review summarizes recent progresses made on NIR-II bioimaging for quantitative analysis. These emergences of various NIR-II fluorescence, photoacoustic (PA), luminescence lifetime imaging probes and their quantitative analysis applications are comprehensively discussed, and perspectives on potential challenges facing in this direction are also raised.
近红外二区（NIR-II）生物成像作为生物过程研究实时调查中的一种新兴可视化方式。由于在近红外二区（1000-1700纳米）降低光子散射和低组织自体荧光水平方面取得的进展，NIR-II生物成像随着组织穿透深度的增加提供高分辨率，并且在检测以获得更详细的定性和定量参数方面显示出更大的应用潜力。在此，本综述总结了NIR-II生物成像在定量分析方面的最新进展。全面讨论了各种NIR-II荧光、光声（PA）、发光寿命成像探针的出现及其定量分析应用，并提出了该方向面临的潜在挑战的观点。
