Truscott W M, Hirsh D C
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Infect Immun. 1988 Jun;56(6):1538-44. doi: 10.1128/iai.56.6.1538-1544.1988.
A strain of Pasteurella multocida of avian origin was found to inhibit phagocytosis of Candida albicans by mononuclear phagocytes in vitro. Whole-cell lysates of P. multocida showed this effect, as did a 50-kilodalton (kDa) protein eluted from sodium dodecyl sulfate-polyacrylamide gels obtained by electrophoresis of whole-cell lysates. Heat, digestion with trypsin, and antibody specific for this 50-kDa protein neutralized the antiphagocytic effects of P. multocida, of the whole-cell lysates, and of the 50-kDa protein itself. Evidence that this protein was in the outer membrane of the bacterial cell included the findings that (i) treatment of encapsulated or unencapsulated P. multocida with trypsin reduced the antiphagocytic effect; (ii) whole-cell lysates prepared from trypsinized, unencapsulated P. multocida had reduced antiphagocytic activity; and (iii) antibody to outer membrane proteins neutralized the antiphagocytic effect. Turkeys given antibodies specific for the 50-kDa outer membrane protein were protected against lethal challenge with P. multocida.
发现一株禽源多杀巴斯德菌在体外可抑制单核吞噬细胞对白色念珠菌的吞噬作用。多杀巴斯德菌的全细胞裂解物显示出这种作用，从全细胞裂解物经电泳获得的十二烷基硫酸钠-聚丙烯酰胺凝胶上洗脱下来的一种50千道尔顿（kDa）的蛋白质也有此作用。加热、用胰蛋白酶消化以及针对这种50-kDa蛋白质的抗体可中和多杀巴斯德菌、全细胞裂解物以及50-kDa蛋白质本身的抗吞噬作用。该蛋白质存在于细菌细胞外膜的证据包括以下发现：（i）用胰蛋白酶处理有荚膜或无荚膜的多杀巴斯德菌可降低其抗吞噬作用；（ii）由经胰蛋白酶处理的无荚膜多杀巴斯德菌制备的全细胞裂解物的抗吞噬活性降低；（iii）外膜蛋白抗体可中和抗吞噬作用。用针对50-kDa外膜蛋白的特异性抗体处理的火鸡可免受多杀巴斯德菌致死性攻击。
