Rush H G, Glorioso J C, DaRif C A, Olson L C
Am J Vet Res. 1981 Oct;42(10):1760-8.
The susceptibilities of several Pasteurella multocida strains to serum bactericidins and to killing by rabbit polymorphonuclear neutrophils (PMN) were investigated, using in vitro assays. Strain Bunia II (serotype 5,12:E) was killed by both immune and normal rabbit sera containing active complement, and strains R1 (serotype 3,12:A) and 656 (serotype 12:B) were serum resistant. Strain R1 opsonized with immune or normal rabbit serum with complement or with immune antibody alone was phagocytized by neutrophils, and strain 656 was ingested only after opsonization with immune antibody in the presence or absence of complement. Complement alone was ineffective as an opsonin for the last 2 serotypes. Neither isolate was resistant to PMN killing. Growth of strain R1 in subcutaneously implanted chambers in rabbits did not increase the resistance of this organism to PMN. Comparison of phagocytosis and killing of this isolate with 2 virulent rabbit strains of P multocida--strains L-A (serotype 12:A) and 7228 (serotype 14:D)--demonstrated that strain L-A was resistant to destruction by neutrophils, whereas strains 7228 and R1 were killed. Resistance of strain L-A was not associated with the hyaluronic acid capsule.
采用体外试验研究了多杀性巴氏杆菌的几个菌株对血清杀菌素以及对兔多形核中性粒细胞（PMN）杀伤作用的敏感性。布尼亚II菌株（血清型5,12:E）可被含有活性补体的免疫兔血清和正常兔血清杀灭，而R1菌株（血清型3,12:A）和656菌株（血清型12:B）具有血清抗性。用含有补体的免疫兔血清或正常兔血清或仅用免疫抗体调理的R1菌株可被中性粒细胞吞噬，而656菌株仅在有或无补体存在的情况下用免疫抗体调理后才能被摄取。单独的补体对后两种血清型作为调理素无效。两种分离株对PMN的杀伤均无抗性。R1菌株在兔皮下植入小室中的生长并未增加该菌对PMN的抗性。将该分离株与多杀性巴氏杆菌的2个强毒株——L-A菌株（血清型12:A）和7228菌株（血清型14:D）的吞噬作用和杀伤作用进行比较，结果表明L-A菌株对中性粒细胞的破坏具有抗性，而7228菌株和R1菌株则被杀死。L-A菌株的抗性与透明质酸荚膜无关。
