多杀巴斯德氏菌87千道尔顿外膜抗原oma87基因的克隆、测序、表达及保护能力
Cloning, sequencing, expression, and protective capacity of the oma87 gene encoding the Pasteurella multocida 87-kilodalton outer membrane antigen.
作者信息
Ruffolo C G, Adler B
机构信息
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
出版信息
Infect Immun. 1996 Aug;64(8):3161-7. doi: 10.1128/iai.64.8.3161-3167.1996.
Abstract
Membrane proteins of Pasteurella multocida have been shown previously to elicit protective immunity. We have identified an 87-kDa outer membrane antigen, Oma87, which is present in all 16 serotypes of P. multocida. The gene encoding this protein was cloned and sequenced and found to have significant similarity to the D15 protective surface antigen of Haemophilus influenzae. Oma87 was localized to the outer membrane of the cell, and proteinase K treatment suggested that the protein is surface exposed. Native and recombinant Oma87 were strongly immunostained by convalescent-phase antiserum, indicating that the protein is expressed in vivo. Specific Oma87 antiserum protected mice against homologous, lethal P. multocida challenge. These results suggest that Oma87 is a protective outer membrane antigen of P. multocida.
摘要
多杀性巴氏杆菌的膜蛋白此前已被证明可引发保护性免疫。我们鉴定出一种87 kDa的外膜抗原Oma87，它存在于多杀性巴氏杆菌的所有16个血清型中。编码该蛋白的基因被克隆并测序，发现与流感嗜血杆菌的D15保护性表面抗原有显著相似性。Oma87定位于细胞的外膜，蛋白酶K处理表明该蛋白暴露于表面。天然和重组的Oma87被恢复期血清强烈免疫染色，表明该蛋白在体内表达。特异性Oma87抗血清可保护小鼠免受同源致死性多杀性巴氏杆菌的攻击。这些结果表明Oma87是多杀性巴氏杆菌的一种保护性外膜抗原。
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