Abdissa Rosa, Haile Woynshet, Fite Akafete Teklu, Beyi Ashenafi Feyisa, Agga Getahun E, Edao Bedaso Mammo, Tadesse Fanos, Korsa Mesula Geloye, Beyene Takele, Beyene Tariku Jibat, De Zutter Lieven, Cox Eric, Goddeeris Bruno Maria
College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Oromia, Ethiopia.
Department of Animal Sciences, University of Florida, Gainesville, Florida, USA.
BMC Infect Dis. 2017 Apr 17;17(1):277. doi: 10.1186/s12879-017-2372-2.
There is paucity of information regarding the epidemiology of Escherichia coli O157: H7 in developing countries. In this study, we investigated the occurrence of E. coli O157: H7 associated with beef cattle at processing plants and at retail shops in Ethiopia.
Various samples were collected from beef cattle at slaughter/processing plants, carcass at retail shops and humans at health centers. E. coli O157: H7 was isolated, identified and characterized for antimicrobial resistance, using standard microbiological methods.
At the processing plants E. coli O157: H7 was detected in 1.89% of fecal, 0.81% of intestinal mucosal swab, 0.54% of skin swab and 0.54% of carcass internal swab samples. At retail shops it was detected in 0.8% of carcass and 0.8% of cutting board swab samples, while all samples from utensils, hands from workers, and fecal and stool samples were negative. All isolates were resistant to Amoxicillin, moderately resistant to Cefoxitine and Nitrofurantoins but susceptible to other antimicrobials tested.
E. coli O157: H7 occurs at low prevalence in beef cattle, and the current sanitary dressing procedures in the processing plants and storage conditions in the retail shops are effective against E. coli O157: H7.
关于发展中国家大肠杆菌O157：H7的流行病学信息匮乏。在本研究中，我们调查了埃塞俄比亚加工厂和零售商店中与肉牛相关的大肠杆菌O157：H7的发生情况。
从屠宰/加工厂的肉牛、零售商店的胴体以及健康中心的人类采集了各种样本。使用标准微生物学方法分离、鉴定大肠杆菌O157：H7并对其进行抗微生物药物耐药性特征分析。
在加工厂，粪便样本中1.89%、肠黏膜拭子样本中0.81%、皮肤拭子样本中0.54%以及胴体内拭子样本中0.54%检测到大肠杆菌O157：H7。在零售商店，胴体样本中0.8%以及砧板拭子样本中0.8%检测到该菌，而来自器具、工人手部以及粪便和大便样本的所有样本均为阴性。所有分离株对阿莫西林耐药，对头孢西丁和呋喃妥因中度耐药，但对其他测试抗菌药物敏感。
大肠杆菌O157：H7在肉牛中的流行率较低，目前加工厂的卫生处理程序和零售商店的储存条件对大肠杆菌O157：H7有效。
