Lynch C, Seth R, Bates D L, Self C H
Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
J Immunoassay. 1988;9(2):179-92. doi: 10.1080/15321818808057039.
A colorimetric enzyme amplification system was used to develop an immunoassay for human calcitonin (hCT) with a sensitivity of 6 pmol/l, and intra- and inter-assay CVs of 12% and 11.8% respectively for the low pool, and 10% and 11.2% for the high pool. The mean recovery of added synthetic hCT (58.5 pmol) from the plasma of 10 patients was 110% (64.4 pmol). The correlation coefficient between radioimmunoassay (RIA) and amplified enzymo-immunoassay was found to be 0.96 (p 0.001). The assay was successfully applied to the measurement of elevated calcitonin levels in plasma from patients with medullary carcinoma of the thyroid (MCT). AEIA offered a reliable and sensitive alternative to RIA for calcitonin determination with the added advantage of convenience as the label employed was much more stable.
采用比色酶扩增系统开发了一种人降钙素（hCT）免疫测定法，其灵敏度为6 pmol/l，低浓度样本批内和批间变异系数分别为12%和11.8%，高浓度样本分别为10%和11.2%。从10名患者血浆中添加的合成hCT（58.5 pmol）的平均回收率为110%（64.4 pmol）。放射免疫分析（RIA）与扩增酶免疫分析之间的相关系数为0.96（p<0.001）。该测定法成功应用于甲状腺髓样癌（MCT）患者血浆中降钙素水平升高的检测。扩增酶免疫分析（AEIA）为降钙素测定提供了一种可靠且灵敏的RIA替代方法，其额外优势在于操作方便，因为所使用的标记物更稳定。
