Carruggio Francesca, Onofri Andrea, Impelluso Carmen, Giusso Del Galdo Gianpietro, Scopece Giovanni, Cristaudo Antonia
Department of Biological, Geological and Environmental Sciences, Germplasm Bank (BGS-CT), University of Catania, 95128 Catania, Italy.
Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, Italy.
Plants (Basel). 2020 Aug 27;9(9):1110. doi: 10.3390/plants9091110.
Most legumes are well-known for the physical dormancy of their seeds; hence, the implementation of appropriate scarification techniques is essential for introducing new legume crops within agricultural systems. This study investigated morpho-anatomical traits and dormancy-breaking requirements in two taxa of the genus the widespread and the point endemic . As the species under investigation show monospermic indehiscent legumes, pods were used in this research. We performed pod trait measurements, light microscopy observations on the seed coat anatomical structure, and germination tests after mechanical, thermal, and chemical scarification treatments for seed dormancy breaking. Moreover, germination performance at different pod maturity stages and storage times was tested. Differences in morpho-anatomical traits were found, with having a thicker palisade cell layer and showing larger pods. All of the scarification treatments proved to be able to break physical dormancy, with mechanical and chemical scarification being the most effective methods in both species. Nevertheless, dormancy-breaking treatments performed better in . Seeds at early pod maturity stages showed higher germination capacity in both species. Overall, this research provided background knowledge on seed collection time, storage strategy, and effective pre-sowing treatment, which might contribute to enhance propagation and use of species for multiple purposes. Under this perspective, the future characterization of additional genetic resources from other Mediterranean populations will have remarkable importance.
大多数豆科植物以其种子的物理休眠而闻名；因此，实施适当的擦伤技术对于在农业系统中引入新的豆科作物至关重要。本研究调查了豆科属两个分类群（广泛分布的[物种名称1]和点状特有种[物种名称2]）的形态解剖特征和打破休眠的要求。由于所研究的物种表现为单粒不开裂的豆科植物，本研究使用了豆荚。我们进行了豆荚性状测量、种皮解剖结构的光学显微镜观察，以及机械、热和化学擦伤处理以打破种子休眠后的发芽试验。此外，还测试了不同豆荚成熟阶段和储存时间的发芽性能。发现了形态解剖特征的差异，[物种名称1]的栅栏细胞层较厚，[物种名称2]的豆荚较大。所有擦伤处理都被证明能够打破物理休眠，机械和化学擦伤是两种物种中最有效的方法。然而，打破休眠的处理在[物种名称2]中表现更好。在两个物种中，早期豆荚成熟阶段的种子发芽能力更高。总体而言，本研究提供了关于种子收集时间、储存策略和有效的播种前处理的背景知识，这可能有助于促进[物种名称]物种的繁殖和多种用途的利用。从这个角度来看，未来对来自其他地中海种群的额外[物种名称]遗传资源进行表征将具有重要意义。
